Alzheimer's disease (AD) is a neurodegenerative disease with characteristics of progressive cognitive impairment

abnormal behavior and personality change. There is no effective treatment method for AD in a global context as AD is a multifactorially complex disease. Intervention to AD is always late. More and more researchers proposed that early intervention and multiple targetsare needed in AD treatment. In the guidance of the principles of nourishing the kidney

strengthening the essence

and prevention before disease onset

we studied the neuroprtective effects of Jiawei Wuzi Yanzong Fang on mild cognitive impairment. Clinic study showed Jiawei Wuzi Yanzong Fang can improve the clinical symtoms of mild cognitive impairment(MCI) patients

delay the development of MCI to AD

the mechanism is related to the inhibition activity of acetylcholine esterase

and reduce the oxidative damage of DNA in mitochondria and the Aβ level in serum. Imageology study also proved that this prescription can prevent and cure the hippocampal volume shrikage as it can reduce the hippocampal index and the temporal angle width. We also used the method of high performance liquid chromatography-photodiode array detector-tandem mass spectrum (HPLC-PDA-MS/MS) to analyze the prescription's compositions. Then we constructed the HPLC Fingerprint of the Jiawei Wuzi Yanzong Fang. Finally

the further experiments in vivo and in vitro confirmed that the Jiawei Wuzi Yanzong Fang and its effective compositions could improve the AD rats models' behavioristics symptomes

regulate the central nervous cholinergic system

inhibit the impairment induced by Aβ

oxidative stress and inflammation. Thus we concluded that the Jiawei Wuzi Yanzong Fang and its effective compositions could exerts neuroprtective effect at multipletargets and in multiplepathways.