FAN Hui, TIAN Yuan. Optimization of Extraction Technology for Tianma Anshen Granules by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 21-23.
DOI:
FAN Hui, TIAN Yuan. Optimization of Extraction Technology for Tianma Anshen Granules by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 21-23. DOI: 10.13422/j.cnki.syfjx.2015070021.
Optimization of Extraction Technology for Tianma Anshen Granules by Orthogonal Design
Objective: To optimize extraction technology of Tianma Anshen granules. Method: With dry extract rate and contents of gastrodin
schizandrin
rhynchophylline as comprehensive index
orthogonal design was adopted to investigate effects of the amount and concentration of ethanol
extracting time and times on extraction technology.Contents of gastrodin
schizandrin and rhynchophylline were determined by HPLC
mobile phase of gastrodin was acetonitrile-0.05% phosphoric acid (3:97)
detection wavelength was 220 nm;mobile phase of schizandrin and rhynchophylline was acetonitrile-0.001% triethylamine (45:55)
detection wavelength was 250 nm. Result: Optimal extraction technology was extracted 3 times with 6 times the amount of 50% ethanol for 1 h per time.Extraction amounts of gastrodin
schizandrin and rhynchophylline were 2.69
1.47
0.18 mg·g-1
dry extract rate was 22.64%. Conclusion: This optimized extraction technology is stable
feasible and suitable for industrial production of Tianma Anshen granules.
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Related Author
ZHANG Menglian
ZHANG Yaowen
TANG Linfeng
GONG Zaipeng
HAN Lan
WANG Dandan
Fan LIU
Ting-ting CHEN
Related Institution
Anhui Province Key Laboratory of Traditional Chinese Medicine,School of Pharmacy, Anhui University of Chinese Medicine
State Key Laboratory of Efficacy and Utilization of Medicinal Plants,Guizhou Medical University
Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University
The Affiliated Hospital of Guizhou Medical University