Optimization of Extraction Technology of Herbs Containing Alkaloids from Compound Tingli Xiefei Lishui Mixture

WANG Li; HUANG Yi-ping; XU Jia; MA Ya-ping; LIU Xiao-min

doi:10.13422/j.cnki.syfjx.2015070027

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Optimization of Extraction Technology of Herbs Containing Alkaloids from Compound Tingli Xiefei Lishui Mixture

更新时间：2024-09-23

- Optimization of Extraction Technology of Herbs Containing Alkaloids from Compound Tingli Xiefei Lishui Mixture
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 7, Pages: 27-30(2015)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2015070027
  CLC： R284.2
- Published：2015
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WANG Li, HUANG Yi-ping, XU Jia, et al. Optimization of Extraction Technology of Herbs Containing Alkaloids from Compound Tingli Xiefei Lishui Mixture[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 27-30.
DOI：

WANG Li, HUANG Yi-ping, XU Jia, et al. Optimization of Extraction Technology of Herbs Containing Alkaloids from Compound Tingli Xiefei Lishui Mixture[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 27-30. DOI： 10.13422/j.cnki.syfjx.2015070027.

摘要

目的: 考察不同提取方法中法半夏对黄连中盐酸小檗碱溶出率的影响。方法: 选择复方葶苈泻肺利水合剂为考察对象

分别以水和乙醇为溶媒

采用方中法半夏、黄连、益母草、半边莲合提取及缺法半夏其余3味药材提取2种方法提取

比较提取液中盐酸小檗碱含量。以盐酸小檗碱提取量为指标

通过正交试验考察乙醇体积分数、提取时间、提取次数及料液比对复方葶苈泻肺利水合剂中4味药材提取工艺的影响。采用HPLC测定盐酸小檗碱含量

流动相乙腈-0.05 mol·L-1磷酸二氢钾溶液(50:50)(每100 mL中加十二烷基硫酸钠0.4 g

以磷酸调节pH 4.0)

检测波长345 nm。结果: 以水为溶媒

2种方式提取的盐酸小檗碱含量差别较大

合提与缺法半夏提取时盐酸小檗碱平均质量分数分别为9.20

13.53 mg·g-1;以乙醇为溶媒

2种方式提取的盐酸小檗碱含量差别较小

平均质量分数分别为35.15

36.12 mg·g-1。4味药材的最佳提取工艺为加10倍量70%乙醇回流提取2次

每次1.5 h;盐酸小檗碱提取量37.92 mg·g-1。结论: 以水为溶媒

法半夏与黄连合提将导致盐酸小檗碱含量显著降低;以乙醇为溶媒

法半夏与黄连合提对盐酸小檗碱溶出率影响较小

且在乙醇溶液中盐酸小檗碱提取率远高于水提法。优选的提取工艺稳定可行、重复性好

可用于复方葶苈泻肺利水合剂的工业化生产。

Abstract

Objective: To investigate effects of Pinelliae Rhizoma Praeparatum on dissolution rate of berberine in Coptidis Rhizoma by different extraction methods. Method: Taking compound Tingli Xiefei Lishui mixture for example

water and ethanol were selected as solvent

extraction methods included mixed extraction of four herbs and remaining three herbs without Pinelliae Rhizoma Praeparatum

then the content of berberine in extract were compared.With extracting amount of berberine as index

orthogonal test was used to optimize extraction technology with the concentration and amount of ethanol

extracting times and time as factors.HPLC was employed to determine the content of berberine with mobile phase of acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate(50:50) and detection wavelength at 345 nm. Result: In water extraction process

the content of berberine was significantly differernt

average contents of berberine from mixed extraction of four herbs and remaining three herbs without Pinelliae Rhizoma Praeparatum were 9.20

13.53 mg·g-1

respectively.In ethanol extraction process

the content of bererine had little difference

which were 35.15

36.12 mg·g-1.Optimum ethanol extraction process was as follows:reflux extracted 2 times with 10 times the amount of 70% ethanol for 1.5 h each time;extracting amount of berberine was 37.92 mg·g-1. Conclusion: With water as solvent

when four herbs mixed extractiing

Pinelliae Rhizoma Praeparatum can reduce the content of bererine significantly.With ethanol as solvent

when four herbs mixed extracting

Pinelliae Rhizoma Praeparatum has little effect on the content of bererine

and ethanol extraction method has higher extracting rate than water extraction method.This optimized technology is stable and feasible with good repeatability

and it can be used in industrial production of compound Tingli Xiefei Lishui mixture.

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Related Institution

School of Chinese Medicine，Chongqing University of Chinese Medicine

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School of Traditional Chinese Medicine（TCM），Chongqing University of Chinese Medicine

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