WEN Zhi-rui, YANG Wei-ling, TIAN Hong-hong, et al. Fingerprints of Volatile Oil from Epimedii Folium by GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 39-42.
DOI:
WEN Zhi-rui, YANG Wei-ling, TIAN Hong-hong, et al. Fingerprints of Volatile Oil from Epimedii Folium by GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 39-42. DOI: 10.13422/j.cnki.syfjx.2015070039.
Fingerprints of Volatile Oil from Epimedii Folium by GC-MS
Objective: To investigate the chromatographic fingerprints of volatile oil from Epimedii Folium by GC-MS. Method: The volatile oil was extracted from Epimedii Folium by steam distillation method
chromatographic fingerprints of which was detected and calculated by GC-FID
and the mutual fingerprints of ten kinds essential oil from Epimedii Folium was established. GC conditions:HP-INNOWAX column inlet temperature 260 ℃
the split ratio of 5:1;temperature program:initial temperature of 60 ℃
keep 10 min
with 2 ℃·min-1 rose to 250 ℃
13 min to maintain. GC-MS parameters:column flow rate 1.0 mL·min-1
MS electron energy 70 eV
ion source temperature of 230 ℃
the quadrupole temperature 180 ℃
the transmission line temperature 280 ℃
full-ion scan mass range m/z 40-550
solvent delay 3 min. GC-FID parameters:column flow rate 1.5 mL·min-1
FID detector temperature 260 ℃
the hydrogen flow rate 30 mL·min-1
the air flow 300 mL·min-1
tail blowing nitrogen 30 mL·min-1. Result: The method was proved to be accuracy
reproducible and stability
by which the ideal fingerprints of volatile oil from Epimedii Folium were obtained. Conclusion: The methods set up are beneficial for the quality control of Epimedii Folium.