Objective: A high-performance liquid chromatography-UV method was developed to determine the contents of oleic acid and linoleic acid in Bruceae Fructus oil. Method: A chromatographic process was set up by using column Ultimate XB-C18(4.6 mm×150 mm

5 μm). A mobile phase of 88% acetonitrile-12% phosphoric acid solution (0.1%) was used with flow rate at 1.0 mL·min-1

column temperature at 30 ℃

and determine wavelength at 203 nm. Result: The calibration curve of oleic acid was linear between 19.219-615.00 mg·L-1

and linoleic acid was linear between 3.375-108.0 mg·L-1

with the average recovery 81.29%-108.44% and RSD of 1.3%-2.9%. The contents of oleic acid and linoleic acid in Bruceae Fructus oil were 68.10% and 12.49%. Conclusion: This new analytical method do not need the operation of pre-column derivatization

and with the advantage of simple

accurate and repeatable

which could be a critical reference for quality control of Bruceae Fructus oil pharmaceutical manufacture.