Reversion of Multi Drug Resistance by Nitidine Chloride on KB/ADM Cell

ZHANG Hua-jun; LIU Hua-gang; LIANG Yan; FENG Yan-ying; LI Jun-wei

doi:10.13422/j.cnki.syfjx.2015070087

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Reversion of Multi Drug Resistance by Nitidine Chloride on KB/ADM Cell

更新时间：2024-09-23

- Reversion of Multi Drug Resistance by Nitidine Chloride on KB/ADM Cell
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 7, Pages: 87-90(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015070087
  CLC： R285.5
- Published：2015
- 稿件说明：
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ZHANG Hua-jun, LIU Hua-gang, LIANG Yan, et al. Reversion of Multi Drug Resistance by Nitidine Chloride on KB/ADM Cell[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 87-90.
DOI：

ZHANG Hua-jun, LIU Hua-gang, LIANG Yan, et al. Reversion of Multi Drug Resistance by Nitidine Chloride on KB/ADM Cell[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 87-90. DOI： 10.13422/j.cnki.syfjx.2015070087.

摘要

目的: 探讨氯化两面针碱(nitidine chloride

NC)对人口腔鳞癌耐阿霉素(ADM)细胞株KB/ADM多药耐药性(MDR)的逆转作用

开发低毒高效的抗肿瘤药物多药耐药逆转剂。方法: 人口腔鳞癌多药耐药细胞KB/ADM常规培养至细胞数为1×106

随机分为多药耐药细胞组(KB/ADM细胞组)

阳性药物维拉帕米组(VRP

5 μmol·L-1)

NC低剂量组(0.375 mg·L-1)

NC中剂量组(0.75 mg·L-1)

NC高剂量组(1.5 mg·L-1)5组

每组3个平行样本。分别加入同体积的培养基

5 μmol·L-1 VRP

0.375

0.75

1.5 mg·L-1 NC处理48 h

MTT法分别检测各组细胞对化疗药物ADM

长春新碱(VCR)

依托泊苷(VP-16)

羟基喜树碱(HCPT)敏感性;流式细胞仪检测P糖蛋白的表达;RT-PCR法检测多药耐药基因-1(MDR1)的表达。结果: 与多药耐药细胞组比较

阳性药物VRP组

NC低、中、高剂量组KB/ADM细胞对ADM

VCR

VP-16

HCPT的IC50值明显下降(P<0.01

P<0.05);阳性药物VRP组

NC低、中、高剂量组KB/ADM细胞P糖蛋白的表达分别为(10.60±1.11)%

(68.57±2.56)%

(28.29±1.98)%

(8.03±1.46)%

与多药耐药细胞组(97.33±1.61)%比较明显下降(P<0.01);阳性药物VRP组、NC低、中、高剂量组分别使KB/ADM的多药耐药性基因(MDR1)相对表达下降39%

22%

46%

69%(P<0.01)。结论: 氯化两面针碱能有效逆转KB/ADM多药耐药性

具有良好的抗肿瘤药物多药耐药逆转作用。

Abstract

Objective: To investigate the reversal effect of nitidine chloride(NC) on human oral squamous cell carcinoma KB/adriamycin(ADM). Method: The KB/ADM cells were cultured conventionally to 1×106

randomly divided into five groups:KB/ADM cells group

positive control verapamil group(VRP

5 μmol·L-1)

low dose group of NC(0.375 mg·L-1)

middle dose group of NC(0.75 mg·L-1)

high dose group of NC(1.5 mg·L-1)

3 parallel samples each.These groups were respectively added to the same volume of culture medium

5 μmol·L-1 VRP

0.375

0.75

1.5 mg·L-1 NC for 48 h.The cell sensitivity to chemotherapeutic drugs was demonstrated by MTT. The effect of NC on p-glyco protein(P-gp) expression was analyzed by flow cytometry. Multidrug resistance gene-1(MDR-1) expression was detected by RT-PCR. Result: Compared with KB/ADM cells group

the IC50 value of positive control VRP group

low dose

middle dose and high dose group of NC to ADM

vincristine(VCR)

estoposide(VP-16)

hydroxycamptothecin(HCPT) were decreased significantly(P<0.01

P<0.05). The P-gp expressions in VRP group

low

middle and high dose group with NC were respectively (10.60±1.11)%

(68.57±2.56)%

(28.29±1.98)%

(8.03±1.46)%

compared with KB/ADM cells group(97.33±1.61)%

all of them were decreased significantly(P<0.01).The MDR1-mRNA expressions of positive VRP group

low dose

middle dose and high dose group with NC decreased by 39%

22%

46%

69%(P<0.01)compared with KB/ADM cells group. Conclusion: NC can reverse the multidrug resistance of KB/ADM.

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