Feixianfang Extract Interfering Vascular Cell Adhesion Molecule 1 and Plasminogen Activator Inhibitor-1 mRNA Expression in Rat Model of Pulmonary Fibrosis

LIU Zhe; ZHANG Xiao-mei; QIN Hui-hui; ZHANG Li-yuan; CHEN Liang; HUANG Yan-fang; GUAN Li-zhen; WANG Qi; JIANG Liang-duo

doi:10.13422/j.cnki.syfjx.2015070107

您当前的位置：

首页 >

文章列表页 >

Feixianfang Extract Interfering Vascular Cell Adhesion Molecule 1 and Plasminogen Activator Inhibitor-1 mRNA Expression in Rat Model of Pulmonary Fibrosis

更新时间：2024-09-23

- Feixianfang Extract Interfering Vascular Cell Adhesion Molecule 1 and Plasminogen Activator Inhibitor-1 mRNA Expression in Rat Model of Pulmonary Fibrosis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 7, Pages: 107-110(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015070107
  CLC： R285.5
- Published：2015
- 稿件说明：
移动端阅览
LIU Zhe, ZHANG Xiao-mei, QIN Hui-hui, et al. Feixianfang Extract Interfering Vascular Cell Adhesion Molecule 1 and Plasminogen Activator Inhibitor-1 mRNA Expression in Rat Model of Pulmonary Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 107-110.
DOI：

LIU Zhe, ZHANG Xiao-mei, QIN Hui-hui, et al. Feixianfang Extract Interfering Vascular Cell Adhesion Molecule 1 and Plasminogen Activator Inhibitor-1 mRNA Expression in Rat Model of Pulmonary Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 107-110. DOI： 10.13422/j.cnki.syfjx.2015070107.

摘要

目的: 探讨肺纤方干预肺间质纤维化炎性反应及纤溶抑制的作用。方法: 将大鼠分为假手术组、模型组、泼尼松组、氯沙坦组、肺纤方提取物高、中、低剂量组(2.4

1.2

0.6 g·kg-1)7组

采用气管插管灌注博莱霉素3 mg·kg-1进行大鼠肺间质纤维化造模。造模第2天予药物灌胃干预。于第14

28天分两批进行动物处理

HE染色观察肺泡炎程度

Masson染色观察肺纤维化程度。RT-PCR检测血管细胞黏附分子(VCAM-1)

纤溶酶原激活物抑制因子(PAI-1)基因表达。结果: 肺纤方中剂量组能够显著抑制肺泡炎、肺纤维化程度

降低VCAM-1

PAI-1mRNA表达。结论: 肺纤方通过抑制VCAM-1

PAI-1基因表达

减轻炎性反应、改善纤溶抑制而发挥抗纤维化作用。

Abstract

Objective: To investigate the intervention of Feixianfang(FXF) in lung inflammatory reaction and fibrosis. Method: Rats were randomly divided into 7 groups:the sham operation group

model group

FXF high dose group

FXF middle dose group

FXF low dose group

the control group of Western medicine was divided into two groups:Losartan group

and prednisone group. Rats were given bleomycin(BLM) to induce model via tracheal intubation

3 mg·kg-1. Intragastric administration started from the next day after modeling. The rats were killed on 14 and 28 days after modeling

respectively. HE staining and Masson staining were applied to determine the degree of the alveolitis and pulmonary fibrosis.RT-PCR were applied to detect plasminogen activator inhibitor-1(PAI-1)

vascular cell adhesion molecule 1(VCAM-1) gene expression. Result: FXF middle dose could inhibit the degree of alveolitis(P<0.05). FXF middle dose can inhibit the degree of pulmonary fibrosis and the gene expression of VCAM-1

PAI-1(P<0.05). Conclusion: FXF improve pulmonary fibrosis by inhibiting expression of VCAM-1 and PAI-1 genet.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰