Anti-metastatic Effects of Extracts from Saururi Herba Downregulating Runx2 Activation on Breast Cancer Cell

LYU Hong; ZOU Le-lan; MA Jun-chao; WANG Shan; LIU Ting; KE Xiao-qin; FU Jian-jiang

doi:10.13422/j.cnki.syfjx.2015070123

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Anti-metastatic Effects of Extracts from Saururi Herba Downregulating Runx2 Activation on Breast Cancer Cell

更新时间：2024-09-23

- Anti-metastatic Effects of Extracts from Saururi Herba Downregulating Runx2 Activation on Breast Cancer Cell
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 7, Pages: 123-127(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015070123
  CLC： R285.5
- Published：2015
- 稿件说明：
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LYU Hong, ZOU Le-lan, MA Jun-chao, et al. Anti-metastatic Effects of Extracts from Saururi Herba Downregulating Runx2 Activation on Breast Cancer Cell[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 123-127.
DOI：

LYU Hong, ZOU Le-lan, MA Jun-chao, et al. Anti-metastatic Effects of Extracts from Saururi Herba Downregulating Runx2 Activation on Breast Cancer Cell[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 123-127. DOI： 10.13422/j.cnki.syfjx.2015070123.

摘要

目的: 研究三白草提取物(extracts from Saururi Herba

ESH)的抗乳腺癌转移作用。方法: 采用尾静脉注射肿瘤细胞法建立实验性转移模型;采用体外细胞黏附、迁移以及侵袭法

观察ESH对乳腺癌细胞系MDA-MB-231细胞的体外黏附、迁移以及侵袭的影响;采用Runx2转录因子分析法和Western blot法

观察ESH对Runx2活性的影响。结果: 给药后

ESH 200

400 mg·kg-1剂量组裸鼠肺组织中人ck19的平均表达量分别为55.85%

42.28%

显著低于空白组(P < 0.01);体外黏附实验表明

ESH可干扰肿瘤细胞与细胞外基质蛋白之间的黏附(P < 0.01);ESH 80

160 g·L-1剂量组的细胞迁移细胞数分别为(106.4±52.4)

(40.4±7.6)个

与空白组相比具有统计学差异(P < 0.01);细胞侵袭实验表明

ESH低、中、高剂量组的侵袭细胞抑制率分别为32.57%

68.04%和71.92%

与空白组相比具有统计学差异(P < 0.01)。Runx2转录因子活性分析以及Western blot实验结果表明

ESH可显著抑制Runx2的磷酸化

从而抑制其转录活性。结论: ESH 具有明显的抗乳腺癌转移作用。抑制细胞内Runx2磷酸化可能是其发挥抗转移作用的机制之一。

Abstract

Objective: The study was conducted to investigate the anti-metastatic effects of extracts from Saururi Herba (ESH) and its mechanisms. Method: Balb/C Nu mice were used to establish in vivo experimental metastasis model by tail-vein injection;in vitro invasion

in vitro migration and in vitro adhesion assay were performed to determine the effects of ESH on metastatic properties of breast cancer cells;Runx2 transcription factor assay and western blot method were used to detect the effects of ESH on activation of Runx2. Result: ESH exhibited significant suppression on the formation of lung metastatic foci of breast cancer in vivo (P < 0.01). Moreover

in vitro adhesion

migration and invasion of MDA-MB-231 cells were inhibited significantly when these cells were incubated with ESH (P < 0.01). It was also shown that ESH had suppression activity of Runx2 and expression of p-Runx2 in breast cancer cells. Conclusion: ESH shows significant anti-metastatic properties. The mechanisms of this traditional Chinese medicine may be associated with suppression of Runx2 activation.

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