Apoptotic Effects and Mechanism of Lycopene on K562 Cell

SHI Qian-ying; FANG Qin; WANG Ji-shi; CHEN Shu-ya; XU Zhan-xue

doi:10.13422/j.cnki.syfjx.2015070133

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Apoptotic Effects and Mechanism of Lycopene on K562 Cell

更新时间：2024-09-23

- Apoptotic Effects and Mechanism of Lycopene on K562 Cell
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 7, Pages: 133-136(2015)
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- DOI：10.13422/j.cnki.syfjx.2015070133
  CLC： R285.5
- Published：2015
- 稿件说明：
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SHI Qian-ying, FANG Qin, WANG Ji-shi, et al. Apoptotic Effects and Mechanism of Lycopene on K562 Cell[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 133-136.
DOI：

SHI Qian-ying, FANG Qin, WANG Ji-shi, et al. Apoptotic Effects and Mechanism of Lycopene on K562 Cell[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 133-136. DOI： 10.13422/j.cnki.syfjx.2015070133.

摘要

目的: 研究番茄红素(lycopene)对人慢性髓系白血病K562细胞凋亡的影响

探讨其凋亡的作用机制。方法: 用不同浓度(0

60 μmol·L-1)的番茄红素作用K562细胞24

72 h

0 μmol·L-1番茄红素为空白组。采用MTT法检测不同浓度的番茄红素作用24

72 h时K562细胞增殖抑制率;Annexin V/PI双染色法检测不同浓度的番茄红素作用48 h时K562细胞凋亡率;RT-PCR法及Western blot法检测不同浓度的番茄红素作用48 h时K562细胞中B细胞淋巴瘤-2(Bcl-2)

半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)mRNA及蛋白的表达。结果: 与空白组比较

细胞增殖抑制率随番茄红素浓度升高而明显升高

细胞凋亡率逐渐明显增加

随番茄红素浓度明显升高

Bcl-2 mRNA及蛋白的表达明显降低

Caspase-3mRNA及蛋白的表达明显升高

均具有统计学差异(P<0.05)。结论: 番茄红素诱导人慢性髓系白血病K562细胞凋亡

其凋亡机制与下调Bcl-2及上调Caspase-3表达有关。

Abstract

Objective: To investigate the induced apoptotic effects and its mechanism of lycopene on human leukemia cell line K562 cells. Method: K562 cells were treated with different concentrations of lycopene (0

60 μmol·L-1). Cell proliferation in K562 was observed by MTT. Cell apoptosis was inspected by Annexin V/PI double staining method. The expressions of Bcl-2 and Caspase-3 were determined using RT-PCR and Western blot. Result: MTT and Annexin V/PI showed that the rates of proliferation inhibition and apoptosis were positively associated with the concentrations of lycopene (P<0.05). RT-PCR and Western blot indicated that the expression of Bcl-2 gene decreased negatively

but the expression of Caspase-3 gene decreased positively associated with concentration of lycopene (P<0.05). Conclusion: Lycopene could induce the apoptosis of K562 cells

which may be associated with lowering expressions of Bcl-2 and enhancing caspase-3 activity.

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