Protective Effect of Yanshou Decoction on Ozone-induced Skin Aging in Rats

LIU Tian-tian; ZHANG Wei; PENG Jin-e; ZU Cheng-zhe; FENG Li-li; WANG Xiang; SUN Yan; CAI Da-yong; WANG Yue-qi

doi:10.13422/j.cnki.syfjx.2015070142

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Protective Effect of Yanshou Decoction on Ozone-induced Skin Aging in Rats

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- Protective Effect of Yanshou Decoction on Ozone-induced Skin Aging in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 7, Pages: 142-147(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015070142
  CLC： R285.5
- Published：2015
- 稿件说明：
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LIU Tian-tian, ZHANG Wei, PENG Jin-e, et al. Protective Effect of Yanshou Decoction on Ozone-induced Skin Aging in Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 142-147.
DOI：

LIU Tian-tian, ZHANG Wei, PENG Jin-e, et al. Protective Effect of Yanshou Decoction on Ozone-induced Skin Aging in Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 142-147. DOI： 10.13422/j.cnki.syfjx.2015070142.

摘要

目的: 明确延寿液对大鼠吸入臭氧所致皮肤衰老的延缓作用

探讨其作用机制。方法: 雄性SD大鼠随机分为6组

分别为正常组

模型组

维生素E组(13.5 mg·kg-1)

延寿液低、中、高剂量组(45

180 mg·kg-1)

每组8只

正常组吸入空气

造模组吸入臭氧(1.2±0.2) mg·L-1 10 h·d-1×45 d染毒

造模的同时给予延寿液。45 d后动脉取血获得血清与血浆

取大鼠两肩胛至两髂骨之间的背部皮肤

检测血浆谷胱甘肽过氧化物酶 (GSH-Px)和过氧化氢酶 (CAT)活性

血清超氧化物歧化酶(SOD)活性和过氧化脂质(LPO)含量

皮肤羟脯氨酸(Hyp)含量

皮肤组织学观察。形态计量表皮厚度、弹性纤维、胶原纤维、网状纤维、肥大细胞颗粒的体积比

比较组间变化的显著性。结果: 与正常组比较

模型大鼠GSH-Px

CAT

SOD活力明显下降(P<0.01)

LPO含量明显升高(P<0.01)

Hyp含量明显降低(P<0.01);表皮变薄

基底细胞减少

胶原纤维排列稀疏

弹性纤维变短

数量减少

网状纤维较少

出现肥大细胞脱颗粒。延寿液低、中、高剂量组均能明显升高GSH-Px

CAT

SOD活力 (P<0.05

P<0.01)

明显降低LPO含量(P<0.01)

明显升高Hyp含量(P<0.05

P<0.01)

胶原纤维、弹性纤维和网状纤维均增多

增加表皮厚度

减少肥大细胞脱颗粒现象。结论: 通过抑制炎症反应、恢复组织结构

延寿液可延缓大鼠吸入臭氧诱导的皮肤衰老。

Abstract

Objective: To obverse the effect of Yanshou decoction on skin aging in rats induceded by inhaling ozone and to explore its mechanism. Method: Fourty-eight male SD rats were randomly divided into six groups:the normal group

the model group

the Vitamin E group (13.5 mg·kg-1)

the low-

medium-

and high-dose Yanshou decoction groups (45

180 mg·kg-1)

8 in each group. Rats in the normal groups were exposed to fresh air. The other rats were exposed to ozone (1.2±0.2) mg·L-1 4 hours daily for consecutive 45 days. Meanwhile

the corresponding medicines were intragastrically administrated once daily for consecutive 45 days. The activity of serum superoxide dismutase (SOD)

plasma glutathion peroxidase (GSH-Px) or plasma catalase (CAT)

the levels of serum lipid peroxidase (LPO) or dermis hydroxyproline (HYP) were detected and the cutaneous histopathology was observed. Morphometry of skin including epidermis thickness

collagen fiber

elastic fiber and reticular fiber ratio of changes in rats were compared. Result: Compared with the normal group

the activity of SOD

the contents of LPO in serum decreased

the activity of GSH-Px and CAT in plasma decreased

the contents of Hyp in skin tissue homogenate decreased in the model group (P<0.01). Moreover

it was discovered that the epidermis was thin

the basal cells decreased

few collagenous fiber was sparsely arranged

elastic fiber was shorter

and mast cell degranulation were present. Compared with the model group

the activity of SOD

the contents of LPO in serum and the activity of GSH-Px

CAT in plasma increased

the contents of Hyp in skin tissue homogenate increased in all doses of Yanshou decoction groups (P<0.01

P<0.05). The contents of soluble collagen

collagen fiber

elastic fiber and reticular fiber increased and the epidermis was thicker in the Yanshou decoction groups. Moreover

Yanshou decoction could prevent the skin mast cells of rats from releasing granules induced by ozone. Conclusion: Yanshou decoction could postpone the process of skin aging in rats inhaling ozone.

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