Optimization of Extraction Process of Total Flavonoids from  by Box-Behnken Design-response Surface Methodology

LI Fang; YANG Pei-min; CAO Guang-shang; LU Shuang

doi:10.13422/j.cnki.syfjx.2015080021

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Optimization of Extraction Process of Total Flavonoids from by Box-Behnken Design-response Surface Methodology

更新时间：2024-01-04

- Optimization of Extraction Process of Total Flavonoids from by Box-Behnken Design-response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 8, Pages: 21-24(2015)
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.2015080021
  CLC： R284.2
- Published：2015
- 稿件说明：
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LI Fang, YANG Pei-min, CAO Guang-shang, et al. Optimization of Extraction Process of Total Flavonoids from by Box-Behnken Design-response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 21-24.
DOI：

LI Fang, YANG Pei-min, CAO Guang-shang, et al. Optimization of Extraction Process of Total Flavonoids from by Box-Behnken Design-response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 21-24. DOI： 10.13422/j.cnki.syfjx.2015080021.

摘要

目的: 优选白花蛇舌草黄酮类成分的提取工艺

为该药材的资源开发提供参考。方法: 以乙醇体积分数、乙醇用量及提取时间为自变量

芦丁、异槲皮苷、槲皮苷提取量的总评"归一值"为因变量

通过Design-Expert 8.0软件对自变量各水平进行多元线性回归和二项式拟合

利用效应面法优选提取工艺并进行预测分析。采用 HPLC-DAD测定芦丁、异槲皮苷、槲皮苷含量

色谱条件为流动相乙腈(A)-0.05%磷酸溶液(B)梯度洗脱

检测波长254 nm。结果: 最佳提取工艺为加14倍量73%乙醇回流提取2次

每次1.5 h;芦丁、异槲皮苷、槲皮苷平均提取量分别为2.529

0.435

0.215 mg·g-1

总评"归一值"实测值0.922 7与预测值偏差-1.24%。结论: 优化的提取工艺稳定可行

预测准确度高

为白花蛇舌草总黄酮的制剂开发提供参考。

Abstract

Objective: To provide a reference for resource development of Oldenlandia diffusa by optimize extraction process of total flavonoids from this medicinal herb. Method: Extraction time

ethanol concentration and amount were selected as independent variables

overall desirability of contents of rutin

isoquercitrin and quercitrin as dependent variable

Box-Behnken design-response surface methodology was used to optimize extraction technology.HPLC-DAD was adopted to determine contents of rutin

isoquercitrin and quercitrin with mobile phase of acetonitrile (A)-0.05% phosphoric acid solution (B) for gradient elution and detection wavelength of 254 nm. Result: Optimal extraction process was as following:extracted 2 times with 14-fold the amount of 73% ethanol for 1.5 h per time.Average extracting amounts of rutin

isoquercitrin and quercitrin were 2.529

0.435

0.215 mg·g-1

respectively.Deviation between the measured and predicted value of overall desirability was -1.24%. Conclusion: This extraction process is stable and feasible with high prediction accuracy

which can provide a reference for preparations development of total flavonoids from O. diffusa.

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