Biotransformation Optimization of 20()-protopanaxadiol by  AS 3.205

CHEN Guang-tong; YANG Xue; GE Hong-juan; LI Jian-lin; SONG Yan

doi:10.13422/j.cnki.syfjx.2015080034

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Biotransformation Optimization of 20()-protopanaxadiol by AS 3.205

更新时间：2024-01-04

- Biotransformation Optimization of 20()-protopanaxadiol by AS 3.205
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 8, Pages: 34-38(2015)
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- DOI：10.13422/j.cnki.syfjx.2015080034
  CLC： Q946
- Published：2015
- 稿件说明：
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CHEN Guang-tong, YANG Xue, GE Hong-juan, et al. Biotransformation Optimization of 20()-protopanaxadiol by AS 3.205[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 34-38.
DOI：

CHEN Guang-tong, YANG Xue, GE Hong-juan, et al. Biotransformation Optimization of 20()-protopanaxadiol by AS 3.205[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 34-38. DOI： 10.13422/j.cnki.syfjx.2015080034.

摘要

目的: 利用微生物转化的方法构建20(S)-原人参二醇过氧衍生物的新合成途径

并对转化条件进行优化

提高过氧衍生物的产率。方法: 选取总状毛霉Mucor racemosus AS 3.205为转化菌株

从接菌量

底物浓度

转化时间

加样时间

温度

转速等方面对转化条件进行优化。结果: 获得2个20(S)-原人参二醇过氧衍生物

20(S)-原人参二醇为25

26-烯-24(R)过氧羟基-20(S)-原人参二醇和23

24-烯-25过氧羟基-20(S)-原人参二醇;获得了优化的转化工艺

即接种量为10%

底物浓度为0.25 mmol·L-1

加样时间为转种后48 h

培养温度为28 ℃

转化时间为5 d

摇床转速为150 r·min-1。结论: 该方法能简便的获得20(S)-原人参二醇过氧衍生物

采用优化后的转化条件25

26-烯-24(R)过氧羟基-20(S)-原人参二醇和23

24-烯-25过氧羟基-20(S)-原人参二醇的产率均明显增大。

Abstract

Objective: The aim of this study was to provide a new approach to synthesize hydroperoxylated products of 20 (S) -protopanaxadiol

and to optimize the microbial transformation conditions by Mucor racemosus AS 3.205. Method: Biotransformation conditions were optimized by altering several influencing factors. The yields of two hydroperoxylated products of 20 (S) -protopanaxadiol were evaluated. Result: Two metabolites were isolated and identified as 25

26-en-24 (R)-hydroperoxyl-20 (S) -protopanaxadiol and 23

24-en-25-hydroperoxyl-20 (S) -protopanaxadiol. The optimal transformation condition was as following:an inoculation amount at 10%

substrate concentration at 0.25 mmol·L-1

sample injected after inoculated 48 h

transformation time of 5 d

culture temperature at 28 ℃

and shaking speed at 150 r·min-1. Conclusion: Microbial transformation system by M. racemosus AS 3.205 can be used to obtain hydroperoxylated products of 20 (S) -protopanaxadiol. The yields of two hydroperoxylated products could be greatly increased under the optimized condition.

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Related Institution

School of Pharmacy，Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine， Henan University of Chinese Medicine

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