QIU Jian-guo, ZHANG Quan-long, LI Mao-xing, et al. Determination of Phenylethanol Glycosides in Different Extract of Lamiophlomis Herba by First Derivative Ultraviolet Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 43-46.
DOI:
QIU Jian-guo, ZHANG Quan-long, LI Mao-xing, et al. Determination of Phenylethanol Glycosides in Different Extract of Lamiophlomis Herba by First Derivative Ultraviolet Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 43-46. DOI: 10.13422/j.cnki.syfjx.2015080043.
Determination of Phenylethanol Glycosides in Different Extract of Lamiophlomis Herba by First Derivative Ultraviolet Spectrophotometry
Objective: To determine the total phenylethanol glycosides in water extract
water eluate and 70% ethanol eluate of Lamiophlomis Herba by first derivative ultraviolet spectrophotometry. Method: Lamiophlomis Herba was extracted with water (18 times) 3 times
1.5 h per time. The water extract was storage at 2-4 ℃
was dried under reduced pressure at 60 ℃. Water extract were separated and enriched by polyamide and macroporous resin
to obtain the water eluate and 70% ethanol eluate Ⅰ (polyamide column)
70% ethanol eluate Ⅱ (macroporous resin column). The total phenylethanol glycosides were determined by first derivative ultraviolet spectrophotometry at 224
252
256
354 nm. Result: Recovery of the total phenylethanol glycosides was 99.71%
RSD was 2.5% at 354 nm. Conclusion: This method is special
sensitive
accurate and repeatable
and can be used for the quality analysis of the total phenylethanol glycosides in Lamiophlomis Herba.
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