Objective: To establish a method for simultaneous determination of liquiritigenin

isoliquiritigenin and licochalcone A in Ganpuquban gel. Method: HPLC was applied on an Agilent HC-C18(4.6 mm×250 mm

5 μm) column by a gradient elution using (A) 0.05% phosphoric acid and (B) acetonitrile as the mobile phase(0-4 min

80%A;4-18 min

80%-65%A;18-22 min

65%-62%A;22-25 min

62% A

25-30 min

62%-55%A;30-35 min

55%A

35-45 min

55%-50%A;45-50 min

50%-55%A;50-60 min

55%-80%A). Detection wavelengths were set at 276

368 nm. The f low rate was 0.8 mL·min-1

the column temperature was maintained at 30 ℃. Result: The linear ranges of liquiritigenin

isoliquiritigenin and licochalcone A were 0.050 4-0.403 2 μg (r=1)

0.039 4-0.315 2 μg (r=1)

1.638-8.192 μg (r=0.999 9). The average recoveries were 98.81% with RSD 1.7%

99.16% with RSD 1.2% and 97.68% with RSD 1.0%

respectively. Conclusion: The established method is easy

accurate with high specificity

which can be used to determine the three components in Ganpuquban gel.