Comparative Analysis of Content of Five Iridoid Glucosides in  Radix from Different Producing Areas

WANG Shuang; DONG Hong-jiao; WANG Chang-sheng; LIU Hai-ping; ZENG Rui

doi:10.13422/j.cnki.syfjx.2015080077

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Comparative Analysis of Content of Five Iridoid Glucosides in Radix from Different Producing Areas

更新时间：2024-01-04

- Comparative Analysis of Content of Five Iridoid Glucosides in Radix from Different Producing Areas
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 8, Pages: 77-80(2015)
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- DOI：10.13422/j.cnki.syfjx.2015080077
  CLC： R284.1
- Published：2015
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WANG Shuang, DONG Hong-jiao, WANG Chang-sheng, et al. Comparative Analysis of Content of Five Iridoid Glucosides in Radix from Different Producing Areas[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 77-80.
DOI：

WANG Shuang, DONG Hong-jiao, WANG Chang-sheng, et al. Comparative Analysis of Content of Five Iridoid Glucosides in Radix from Different Producing Areas[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 77-80. DOI： 10.13422/j.cnki.syfjx.2015080077.

摘要

目的: 建立HPLC同时测定麻花秦艽中马钱苷酸、龙胆苦苷、獐牙菜苦苷、獐牙菜苷、山栀苷甲酯含量的方法。方法: 采用Diamonsil C18色谱柱(4.6 mm× 250 mm

5 μm)

流动相乙腈-0.05%磷酸水梯度洗脱

流速1.0 mL·min-1

柱温30 ℃

检测波长240 nm。结果: 以上5种环烯醚萜类成分的线性范围分别为0.042 1~8.420 0(r=0.999 7)

0.094 2~18.830 0(r=1.000 0)

0.012 4~2.471 2(r=0.999 9)

0.009 9~1.971 2(r=1.000 0)

0.011 3~2.260 0 μg (r=0.999 0)

平均回收率均在95.39%~103.75%

RSD≤2.6%。结论: 该法简便、快速、准确

可以用来同时测定麻花秦艽中5种环烯醚萜苷成分的含量。

Abstract

Objective: To establish an HPLC method for the determination of loganic acid

gentiopicroside

swertiamarin

sweroside

shanzhiside methylester in Gentiana straminea. Method: The assay was performed on a Diamonsil C18 column (4.6 mm× 250 mm

5 μm) eluted with acetonitrile-0.05% phosphoric. The detection wavelength was 240 nm

the flow rate was 1.0 mL·min-1

and the column temperature was set at 30 ℃. Result: The calibration curves were linear in the ranges of 0.042 1-8.420 0 μg(r=0.999 7) for loganic acid

0.094 2-18.830 0 μg(r=1.000 0) for gentiopicroside

0.012 4-2.471 2 μg(r=0.999 9) for swertiamarin

0.009 9-1.971 2 μg (r=1.000 0) for sweroside

0.011 3-2.260 0 μg(r=0.999 0) for shanzhiside methylester. The average recoveries were 95.39%-103.75%(RSD≤2.6%). Conclusion: The method is simple and accurate for the quality control of G. straminea Radix.

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