Objective: To establish an HPLC method for the determination of rutin

hyperoside

quercitrin

quercetin

kaempferol and isorhamnetin in Rhododendron anthopogonoides

while using principal component analysis to evaluate its quality. Method: Samples were extracted with 75% methanol by ultrasound. The separation was performed on a C18 column (4.6 mm×250 mm

5 μm)with acetonitrile-0.2%H3PO4 as mobile phase in a gradient elution. The flow rate was 1.0 mL·min-1

the wavelength was set at 365 nm. Result: Rutin

hyperoside

quercitrin

quercetin

kaempferol

and isorhamnetin have good linearity in the range of 0.009-0.194 9

0.007 1-0.141 6

0.0.188 0-0.375 2

0.027 1-0.434 0

0.010 8-0.216 0

0.005 8-0.116 6 μg

respectively. The recoveries were 100%-105%

RSD were < 3%. Conclusion: The established HPLC method was simple

accurate

reproducible

and reliable. Depending on the chemical indicators

using principal component analysis can be carried out quality assessment for R. anthopogonoides.