LIU Xue-lan, SUN Chang-sheng, ZHU Hong, et al. Influence of Temperature on Quality of Dendrobii Officinalis Caulis Tissue Culture Seedlings[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 89-92.
DOI:
LIU Xue-lan, SUN Chang-sheng, ZHU Hong, et al. Influence of Temperature on Quality of Dendrobii Officinalis Caulis Tissue Culture Seedlings[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 89-92. DOI: 10.13422/j.cnki.syfjx.2015080089.
Influence of Temperature on Quality of Dendrobii Officinalis Caulis Tissue Culture Seedlings
Objective: To investigate influence of temperature on quality of Dendrobii Officinalis Caulis tissue culture seedlings and provide a reference for artificial cultivation of this medicinal herb. Method: Dendrobii Officinalis Caulis tissue culture seedlings at multiplicative and rooting stage were setted in the environment of humidity
light intensity and illumination time controlled
setting temperature were 15
20
25
30
35 ℃
growth conditions of tissue culture seedlings were observed.Pearson correlation was analyzed
variance analysis was adopted to analyze growth index difference among each temperature. Result: Tissue culture seedlings's quality was good at 25-30 ℃ and they showed good growth and reproductive capacity.Pearson correlation analysis showed that temperature with seedling number and plant height were significantly correlated at 0.01 level in proliferation stage;temperature with plant height
leaf number and root number were significantly correlated at 0.01 level in rooting stage.LSD multiple comparison showed that difference of seedling number and plant height were obvious in proliferation stage
difference of leaf number
root number and plant height were obvious in rooting stage. Conclusion: Temperature is the key factor for quality of Dendrobii Officinalis Caulis tissue culture seedlings and the most suitable temperature is 25 ℃.
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