XUE Xiao-xing, LI Yu-bo, LIAN Hong-jian, et al. Research of Relative indexes of Animal Models of Hypertension with Liver Fire Hyperactivity Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 97-101.
DOI:
XUE Xiao-xing, LI Yu-bo, LIAN Hong-jian, et al. Research of Relative indexes of Animal Models of Hypertension with Liver Fire Hyperactivity Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 97-101. DOI: 10.13422/j.cnki.syfjx.2015080097.
Research of Relative indexes of Animal Models of Hypertension with Liver Fire Hyperactivity Syndrome
Objective: To investigate the biological basis of hypertension with hyperactivity of liver fire of macro characterization and behavioral tests
micro physicochemical indexes
disproof prescriptions. Method: Fifty-five SHR rats with hyperactivity of liver fire were divided into model group
Sancao Jiangya decoction (SCD) group
Sini San decoction (SND) group and nifedipine group according to Latin square method
Wistar-Kyoto (WKY) rats as control group. The macro characterization
behavioral indexes and the systolic blood pressure (SBP) were collected. Enzyme-linked immunosorbent assay(ELISA) was used to detect the serum concentration of 5-hydroxytryptamine(5-HT)
angiotensin Ⅱ (ANG Ⅱ)
norepinephrine (NE) after the rats were administrated for 4 and 6 weeks. Result: Compared with control group
the SBP and irritable degree increased
revolving tolerance time decreased
and serum 5-HT
ANGⅡ
NE level was higher in model group. Compared with model
the SBP in SCD group and nifedipine group reduced after treated for 4
6 weeks
(18
20 weeks of age)
the SBP in SND group was stable and increased in the 16 weeks
and then decreased. The symptoms of liver fire hyperactivity improved
and it was not obvious in SND group
and serum 5-HT
ANG Ⅱ
NE level was lower in SCD group compared with model group. Conclusion: Fourteen-eighteen weeks is the stable time window of hyperactivity of liver fire. SHR
5-HT
ANG Ⅱ
NE may be used as the objective index of hypertension of hyperactivity of liver fire syndrome.
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