Effects of Ethanol Extract from  on Growth and Angiogenesis of Human Hepatocellular Carcinoma Xenografts in Nude Mice

OU Ming-chun; SUN Yue-wen; LIU Bu-ming; TANG An-zhou; LIANG Gang

doi:10.13422/j.cnki.syfjx.2015080106

您当前的位置：

首页 >

文章列表页 >

Effects of Ethanol Extract from on Growth and Angiogenesis of Human Hepatocellular Carcinoma Xenografts in Nude Mice

更新时间：2024-01-04

- Effects of Ethanol Extract from on Growth and Angiogenesis of Human Hepatocellular Carcinoma Xenografts in Nude Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 8, Pages: 106-110(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015080106
  CLC： R285.5
- Published：2015
- 稿件说明：
移动端阅览
OU Ming-chun, SUN Yue-wen, LIU Bu-ming, et al. Effects of Ethanol Extract from on Growth and Angiogenesis of Human Hepatocellular Carcinoma Xenografts in Nude Mice[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 106-110.
DOI：

OU Ming-chun, SUN Yue-wen, LIU Bu-ming, et al. Effects of Ethanol Extract from on Growth and Angiogenesis of Human Hepatocellular Carcinoma Xenografts in Nude Mice[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 106-110. DOI： 10.13422/j.cnki.syfjx.2015080106.

摘要

目的: 探讨裂果薯乙醇提取物对人肝癌裸鼠移植瘤生长与肿瘤血管生成的影响及其抗肿瘤的作用机制。方法: 采用80%乙醇回流提取制备裂果薯块茎醇提物;建立人肝癌细胞SMMC-7721裸鼠移植瘤模型

成瘤BALB/c裸鼠随机分为模型组、索拉非尼组和裂果薯醇提物高、中、低剂量组;裂果薯高、中、低剂量组和索拉非尼组给药量分别为107

30 mg·kg-1·d-1

每日ig给药1次

连续给药21 d

模型组不给药

对比观察裂果薯醇提物对肿瘤生长的影响;免疫组化法测定裸鼠肿瘤中CD34

血管内皮生长因子(VEGF)的表达

观察裂果薯醇提物对肿瘤血管生成的影响。结果: 与模型组相比

裂果薯醇提物高、中剂量组和索拉非尼组能够明显降低肿瘤瘤重和相对体积

其抑瘤率分别为47.71%

21.27%

51.75%;同时

裂果薯高剂量组、索拉非尼组能明显降低肿瘤微血管密度(MVD)

(P<0.01)

明显降低肿瘤组织中VEGF的表达(P<0.01);裂果薯中剂量组降低MVD(P<0.05)。结论: 裂果薯醇提物可抑制SMMC-7721细胞裸鼠移植瘤和瘤组织中血管生长

其作用可能与下调VEGF的表达有关。

Abstract

Objective: To investigate the effects of the ethanol extract from Schizocapsa plantaginea on the growth and angiogenesis of SMMC-7721 cell xenografts in nude mice and the antitumor mechanism of S. plantaginea. Method: S. plantaginea tubers were extracted by reflux in 80% ethanol. The human hepatocellular carcinoma SMMC-7721 cell model was established in BALB/c nude mice and the animals were randomly divided into model group

sorafenib group and high

medium

low dosage of S. plantaginea groups. The animals in S. plantaginea groups were administered 107

54 mg·kg-1·d-1 the ethanol extract and in sorafenib group were administered 30 mg·kg-1·d-1sorafenib by gavage once daily for 21 days. The animals in model group did not perform any treatment. The tumor growth was observed in xenografts model in nude mice and angiogenesis was observed by examining the expression of CD34 and vascular endothelial growth factor (VEGF) in tumor tissues by immunohistochemistry. Result: The tumor weight and the relative tumor volume in high and medium dosage of S. plantaginea groups and sorafenib group was significantly lower than that in model group. And the tumor inhibitory rates of those three groups were 47.71%

21.27% and 51.75%. The levels of microvessel density (MVD) and VEGF in tumor tissue were also significantly lower (P<0.01) in high dosage of S. plantaginea group and sorafenib group than in model group. The MVD in medium dosage of S. plantaginea group was less (P<0.05) than that in model group. Conclusion: The ethanol extract from S. plantaginea can inhibit the growth of SMMC-7721 cell xenografts in nude mice and angiogenesis in tumor tissue

and its underlying mechanism is maybe due to its ability to reduce the expression of VEGF.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Venom on Angiogenesis in Rats with Collagen-induced Arthritis

Mechanism of Kidney-tonifying and Liver-regulating Cyclical Therapy and Formula in Improving Endometrial Receptivity during "Implantation Window" in Rats with Polycystic Ovary Syndrome via miR-140-5p/VEGF Pathway

Inhibition of Angiogenesis by Sanguisorbae Radix and Sophorae Flos in Ulcerative Colitis Mice by Regulating PI3K/Akt Signaling Pathway

Protective Effect of Taohong Siwutang on Cerebral Ischemia-reperfusion Injury Based on A1/A2 Phenotype Transformation of Astrocytes Mediated by JAK2/STAT3 Pathway

Modified Shaofu Zhuyutang Mediates VEGF/PI3K/Akt/eNOS Signaling Pathway to Inhibit Angiogenesis in Endometriosis

Related Author

TAO Fang-fang

WANG Li-pei

CHU Li-sheng

QU Tie-bing

LI Lin

LIU Quan

HE Yiqing

ZHANG Ying

Related Institution

Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases， Hunan University of Chinese Medicine

Hunan University of Chinese Medicine

Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine

Affiliated Hospital of Nanjing University of Chinese Medicine

MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰