Study on Analgesic Mechanisms in CNS with Extraction Part of National Medicine

LU Qi-zhen; HUANG Cong; DENG Wei

doi:10.13422/j.cnki.syfjx.2015080141

您当前的位置：

首页 >

文章列表页 >

Study on Analgesic Mechanisms in CNS with Extraction Part of National Medicine

更新时间：2024-01-04

- Study on Analgesic Mechanisms in CNS with Extraction Part of National Medicine
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 8, Pages: 141-144(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015080141
  CLC： R285.5
- Published：2015
- 稿件说明：
移动端阅览
LU Qi-zhen, HUANG Cong, DENG Wei. Study on Analgesic Mechanisms in CNS with Extraction Part of National Medicine [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 141-144.
DOI：

LU Qi-zhen, HUANG Cong, DENG Wei. Study on Analgesic Mechanisms in CNS with Extraction Part of National Medicine [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 141-144. DOI： 10.13422/j.cnki.syfjx.2015080141.

摘要

目的: 探讨紫金莲醇提物2种萃取部位对甲醛致痛大鼠模型的镇痛作用及机制。方法: 56只SD大鼠随机分6组

每组8只

分别设为正常组

模型组

紫金莲醇提物乙酸乙酯部位高(乙高组30 mg·kg-1)、低(乙低组15 mg·kg-1)剂量组

紫金莲醇提物正丁醇部位高(正高组

15 mg·kg-1)、低(正低组

8 mg·kg-1)剂量组。各给药组按剂量ig大鼠

给药7 d

每日1次。第7天末次给药40 min后

除正常组外

其余各组大鼠左后足跖sc 10%甲醛溶液50 μL/只

建立大鼠甲醛致痛模型。观察紫金莲2种活性部位对甲醛致痛模型大鼠痛行为的影响

生化法检测大鼠血清谷氨酸和Ca2+含量

免疫组化法检测大鼠脑和脊髓c-fos阳性细胞表达和P物质(SP)含量。结果: 与正常组比较

模型组大鼠表现出了典型的两时相痛行为学反应

且在脑和脊髓中检测到了大量的c-fos阳性细胞

P物质(SP)

升高血清谷氨酸及Ca2+含量有显著性差异(P<0.05)

表示造模成功。与模型组比较

紫金莲乙低组(15 mg·kg-1)和正低组(8 mg·kg-1)

对大鼠第一时相伤害反应疼痛有显著的抑制作用(P<0.05)

使大鼠血清谷氨酸和脑c-fos蛋白表达显著下降(P<0.05)

血清Ca2+含量显著升高(P<0.05);紫金莲醇提物乙高组(30 mg·kg-1)和正高组(15 mg·kg-1)显著降低大鼠脊髓c-fos蛋白表达(P<0.05);紫金莲醇提物乙高、乙低组和紫金莲醇提物正高、正低组都能显著降低SP在大鼠脑和脊髓中的表达(P<0.05)。结论: 紫金莲醇提物2种萃取部位对大鼠甲醛致痛模型均有镇痛作用

其镇痛机制可能与抑制体内谷氨酸的升高

抑制细胞内Ca2+浓度升高及降低c-fos

SP在中枢神经系统内的表达有关。

Abstract

Objective: To study the analgesic mechanisms of two extraction part of Ceratostigma willmottianum(CW)in rat pain model induced by formaldehyde. Method: Fifty-six SD rats were randomly divided into 6 groups with 8 in each group

including normal group

model group

CW alcohol extract's ethyl acetate part group(30

15 mg·kg-1)

n-butanol part group(15

8 mg·kg-1). Each group was given corresponding drugs by intragastric injection

respectively

once a day for 7 days. At the 7th day

the animals were fasted for 12 hours

and ig 40 min later

except normal group

the other groups were given subcutaneous injection of 10% formaldehyde solution 50 μL for establishing the pain models. The impact of CW active site was observed and serum glutamate and Ca2+ were detected with biochemical kit

and the expression of c-fos and substance P(SP) was assayed with immunohistochemeistry(IHC). Result: Compared with normal group

model group showed a typical pain behavior

amounts of c-fos and SP were detected in the brain and spinal cord

and raised serum glutamic acid concentration

Ca2+ content with significant difference (P<0.05)

meaning that the pain model of rats induced by formaldehyde was successful.Compared with model group

the low dose of ethyl acetate part of CW alcohol extract(15 mg·kg-1) and n-butanol part(8 mg·kg-1)can obvious inhibition to the first phase damage response(P<0.05)

make serum glutamic content

brain c-fos protein expression decreased significantly(P<0.05)

serum Ca2+increased significantly(P<0.05);the high dose of two parts decreased the expression of c-fos in spinal dorsal cord significantly(P<0.05);both of high and low dose of CW alcohol extract's ethyl acetate and n-butanol part decreased the expression of SP significantly(P<0.05)in central nervous system(CNS). Conclusion: Two extraction parts of CW alcohol extract have exact analgesic action in rat pain model induced by formaldehyde

and its analgesic mechanisms may be related to inhibiting the intracellular Ca2+ concentration and glutamate content in the body

reducing the expression of c-fos and SP in CNS.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Application of Natural Ingredients of Traditional Chinese Medicine in Pain Management： A Review

Anti-tumor Effect of Chinese Herbal Polysaccharides： A Review

Mechanism of Chinese Medicine Against Intestinal Mucosal Barrier Injury in Acute Pancreatitis： A Review

A study of comparative pharmacology of Regulating Vital Energy to Alleviate Pain andPromoting Blood Circulation to Stop Pain on Analgesic Effect in Mice(Ⅰ)——The analgesic effect study of Jinlingzisan and Shixiaosan

Study on Analgesic Effect of Duliang Pill(DLP) Extract and Its Effect on C-fos Gene Expression of Periphery Tissue

Related Author

TANG Mingze

SHEN Zhen

GAO Tianle

HAN Yanxing

JIANG Jiandong

Ailing ZHOU

Duanheng WANG

Xiaolei YUE

Related Institution

State Key Laboratory of Bioactive Substances and Function of Natural Medicine，Institute of Materia Medica， Chinese Academy of Medical Sciences and Peking Union Medical College

Guangxi University of Chinese Medicine

The First Clinical Medical College of Shaanxi University of Traditional Chinese Medicine（TCM）

Affiliated Hospital of Shaanxi University of TCM

Cangzhou Medical College

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰