Objective: To observe and screen anti-inflammatory fractions from Jiedu Huoxue Ziyin Recipe (JHZR). Method: The anti-inflammatory effects were observed by series of experiments including mouse auricular edema induced by xylen

increased vascular permeability induced by acetic acid in mice

paw edema caused by carrageen glue in mice

granuloma caused by implanted cotton ball and adjuvant arthritis (AA) in rats. Every experiment included 8 groups:the normal group

the model group

the indomethacin group (10 mg·kg-1) and the 5 groups of different fractions from JHZR (the water extract 966 mg·kg-1

the diethyl ether extract 59 mg·kg-1

the ethyl acetate extract 188 mg·kg-1

the n-butyl alcohol extract 22 mg·kg-1 and the ethyl alcohol extract 321 mg·kg-1). The effect on acute

subacute

and chronic inflammations of these extracts were observed. The effect on serum interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) by JHZR in AA rats were observed. Result: Compared with the model group

the water and ethyl alcohol extracts of JDZR could inhibit the increase of mouse auricular edema by xylen

and the vascular permeability induced by acetic acid

and paw edema caused by carrageen glue

and granuloma caused by implanted cotton ball. Moreover

they could significantly reduce the activities of IL-6 and TNF-α in rats' serum. Conclusion: The water and ethyl alcohol fractions from JDZR have significant anti-inflammatory activity. The former fraction is effective on chronic inflammation

and the latter is effective on acute inflammation.