Objective: To establish a preparation technology of β-cyclodextrin (β-CD) inclusion of volatile oil from Piperis Fructus and to analysis of volatile components by GC-MS. Method: β-CD inclusion of volatile oil from Piperis Fructus was prepared by saturated aqueous solution method

with ratio of β-CD to volatile oil from Piperis Fructus

inclusion temperature and time as factors

comprehensive score of oil content in inclusion

inclusion rate of volatile oil and inclusion yield as index

inclusion conditions were optimized by uniform design.Inclusion was verified through microscopic examination and thermo-gravimetric analysis.GC-MS was employed to compare chemical compositions and percentages of samples which included volatile oil from Piperis Fructus

β-CD inclusion and redistilled volatile oil. Result: Optimum preparation conditions were as follows:β-CD to volatile oil ratio of 6:1

inclusion time of 4 h

inclusion temperature at 60 ℃.Oil content in inclusion

inclusion rate of volatile oil and inclusion yield were 11.56%

75.32% and 86.13%

respectively.These were 46

40 and 45 chemical components were identified from volatile oil

β-CD inclusion and redistilled volatile oil

respectively.But compositions in these samples were very similar

proportions of six main components in total contents of samples were more than 70%.There were some new chemicals appeared and some chemicals disappeared after inclusion and being redistilled

but the total content of these compounds was less than 1%

so that major components of each sample were almost the same. Conclusion: This inclusion process is simple and feasible for production and quality evaluation of solidified powder of volatile oil from Piperis Fructus.