Optimization of Extraction Process of Atractylenolide I,Ⅱ,Ⅲ from Atractylodis Macrocephalae Rhizoma

WANG Jian-cheng

doi:10.13422/j.cnki.syfjx.2015090028

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Optimization of Extraction Process of Atractylenolide I,Ⅱ,Ⅲ from Atractylodis Macrocephalae Rhizoma

更新时间：2024-01-04

- Optimization of Extraction Process of Atractylenolide I,Ⅱ,Ⅲ from Atractylodis Macrocephalae Rhizoma
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 9, Pages: 28-30(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015090028
  CLC： R284.2
- Published：2015
- 稿件说明：
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WANG Jian-cheng. Optimization of Extraction Process of Atractylenolide I,Ⅱ,Ⅲ from Atractylodis Macrocephalae Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(9): 28-30.
DOI：

WANG Jian-cheng. Optimization of Extraction Process of Atractylenolide I,Ⅱ,Ⅲ from Atractylodis Macrocephalae Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(9): 28-30. DOI： 10.13422/j.cnki.syfjx.2015090028.

摘要

目的: 优选白术中3种内酯类成分的提取工艺. 方法: 采用HPLC测定白术内酯Ⅰ

Ⅱ

Ⅲ含量

流动相乙腈(A)-水(B)梯度洗脱(0~16 min

60%~76%A;16~18 min

76%~100%A;18~30 min

100%A)

双波长检测 220 nm(白术内酯Ⅰ

Ⅲ)和276 nm(白术内酯Ⅱ).以白术内酯Ⅰ

Ⅱ

Ⅲ提取率为指标

通过单因素试验筛选提取方式

利用正交试验考察乙醇体积分数、溶剂用量、提取时间及提取次数对白术提取工艺的影响. 结果: 最佳提取方式为乙醇温浸法

最佳工艺条件为加6

4倍量90%乙醇于40 ℃温浸提取3次

提取时间依次为1.5

1.0

1.0 h.白术内酯Ⅰ

Ⅱ

Ⅲ的提取率分别为92.62%

92.39%

93.06%. 结论: 优选的提取工艺稳定可行

白术中3种内酯类成分的提取率高.

Abstract

Objective: To optimize extraction technology of atractylenolide I

Ⅱ

Ⅲ from Atractylodis Macrocephalae Rhizoma. Method: HPLC was adopted to determine contents of atractylenolide I

Ⅱ

Ⅲ with mobile phase of acetonitrile (A)-water (B) for gradient elution (0-16 min

60%-76%A;16-18 min

76%-100%A;18-30 min

100%A) and dual-wavelength detection of 220 nm for atractylenolide I

Ⅱ and 276 nm for atractylenolide Ⅱ.Taking extracting rates of atractylenolide I

Ⅱ

Ⅲ as indexs

extraction method was screened by single factor test

orthogonal test was adopted to optimize extraction process with extracting time

extracting times

ethanol concentration and amount as factors. Result: Alcohol warm infusion method was selected

optimum technological conditions for extracting thrice by adding 6

4 times the amount of 90% ethanol at 40 ℃

extracting time was 1.5

1.0

1.0 h in turn.Extracting rates of atractylenolide I

Ⅱ

Ⅲ were 92.62%

92.39%

93.06%

respectively. Conclusion: This optimized extraction process is stable and feasible with high extraction efficiency of atractylenolide I

Ⅱ

Ⅲ in Atractylodis Macrocephalae Rhizoma.

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