Optimization of Extraction Process of Four Flavonoids in Scutellariae Radix by Central Composite Design-response Surface Methodology

LI Rong; WANG Hai-rong; OU Xiao-qun; LI Xiao-bin; YANG Qiu-nan; XIAO Chang-bin; WAN Li

doi:10.13422/j.cnki.syfjx.2015090035

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Optimization of Extraction Process of Four Flavonoids in Scutellariae Radix by Central Composite Design-response Surface Methodology

更新时间：2024-01-04

- Optimization of Extraction Process of Four Flavonoids in Scutellariae Radix by Central Composite Design-response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 9, Pages: 35-38(2015)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2015090035
  CLC： R284.2
- Published：2015
- 稿件说明：
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LI Rong, WANG Hai-rong, OU Xiao-qun, et al. Optimization of Extraction Process of Four Flavonoids in Scutellariae Radix by Central Composite Design-response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(9): 35-38.
DOI：

LI Rong, WANG Hai-rong, OU Xiao-qun, et al. Optimization of Extraction Process of Four Flavonoids in Scutellariae Radix by Central Composite Design-response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(9): 35-38. DOI： 10.13422/j.cnki.syfjx.2015090035.

摘要

目的: 优选黄芩中4种黄酮类成分的提取工艺

为黄芩薄层鉴别用对照提取物的研究提供参考. 方法: 以乙醇体积分数、溶媒用量、提取时间为自变量

黄芩苷、汉黄芩苷、黄芩素、汉黄芩素提取率的总评“归一值”为因变量

通过星点设计-效应面法优选黄芩提取工艺并进行预测分析和工艺验证.采用HPLC测定指标成分含量

流动相乙腈(A)-0.1%磷酸水(B)梯度洗脱(0~25 min

27%A;25~27 min

27%~43%A;27~40 min

43%~56%A;40~45 min

56%~85%A)

检测波长274 nm. 结果: 最佳提取工艺为加12倍量54%乙醇回流提取2次

每次135 min.黄芩苷、汉黄芩苷、黄芩素和汉黄芩素平均提取率分别为12.53%

2.77%

0.66%

0.25%

总评“归一值”预测值和实测值偏差1.19%. 结论: 优选的提取工艺稳定可行、预测性良好

为黄芩资源的合理利用提供参考.

Abstract

Objective: To optimize extraction process of four flavonoids in Scutellariae Radix and provide references for control extracts research of thin-layer chromatography of Scutellariae Radix. Method: Effects of ethanol concentration

solvent dosage and extracting time on overall desirability of yields of baicalin

wogonoside

baicalein and wogonin were investigated and forecasted by central composite design-response surface methodology.HPLC was adopted to determine contents of four flavonoids with mobile phase of acetonitrile-0.1% phosphoric acid solution and detection wavelength at 274 nm. Result: Optimal extraction process was as following:extracted twice with 12 times the amount of 54% ethanol for per time of 135 min.Yields of baicalin

wogonoside

baicalein and wogonin were 12.53%

2.77%

0.66%

0.25%

respectively.Bias between the predicted and observed value of overall desirability was 1.19%. Conclusion: This optimized extraction technology is stable and feasible with good prediction.

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