Objective: To establish an HPLC method for content determination of magnoflorine

protopine

coptisine and chelidonine in Tong'an injection. Method: Gemini C18(4.6 mm×250 mm

5 μm)was used with the mobile phase of acetonitrile-0.02 mol·L-1 monopotassium phosphate at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 230 nm for magnoflorine

290 nm for protopine and chelidonine

360 nm for coptisine

and the column temperature was maintained at 30 ℃. Result: Calibration curves of the magnoflorine

protopine

chelidonine and coptisine in Tong'an injection showed good linear relationship:10.856-108.560(r=0.999 8)

24.712-247.120(r=0.999 6)

3.464-34.640(r=0.999 6)

39.296-392.960 mg·L-1 (r=0.999 9). The average recoveries of the components above were between 97.5%-101.8%. Conclusion: The method was feasible

accurate and stable

which could be used for determination of magnoflorine

protopine

coptisine and chelidonine in Tong'an injection.