Effect of Kansui Radix Combining with Glycyrrhizae Radix et Rhizoma in Rat's Kidney by Metabonomics

TANG Bing-wen; LI Jun-jian; WU Fu-hai; SONG Fen-yun

doi:10.13422/j.cnki.syfjx.2015090088

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Effect of Kansui Radix Combining with Glycyrrhizae Radix et Rhizoma in Rat's Kidney by Metabonomics

更新时间：2024-01-04

- Effect of Kansui Radix Combining with Glycyrrhizae Radix et Rhizoma in Rat's Kidney by Metabonomics
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 9, Pages: 88-92(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015090088
  CLC： R285.5
- Published：2015
- 稿件说明：
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TANG Bing-wen, LI Jun-jian, WU Fu-hai, et al. Effect of Kansui Radix Combining with Glycyrrhizae Radix et Rhizoma in Rat's Kidney by Metabonomics[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(9): 88-92.
DOI：

TANG Bing-wen, LI Jun-jian, WU Fu-hai, et al. Effect of Kansui Radix Combining with Glycyrrhizae Radix et Rhizoma in Rat's Kidney by Metabonomics[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(9): 88-92. DOI： 10.13422/j.cnki.syfjx.2015090088.

摘要

目的: 根据传统用药习惯

从代谢组学的角度来验证甘遂-甘草以1: 4比例配比时对大鼠肾脏的减毒作用

并在此基础上初步探讨其减毒作用机制. 方法: 实验采用SD雄性大鼠

随机分为空白对照组

甘遂组

甘草组和甘遂-甘草1: 4组.应用核磁共振技术(NMR)和正交-偏最小二乘判别分析(OPLS-DA)方法分析给予大鼠甘遂、甘草和甘遂-甘草1: 4配比液后的大鼠肾脏代谢物变化

并结合血浆生化分析和肾脏组织病理切片HE染色检测

明确甘遂-甘草1: 4配伍的减毒作用机制. 结果: 甘草组极少引起血浆生化指标和病理学改变

同时对大鼠肾脏内源性代谢物的变化不大;甘遂组能引起血浆葡萄糖的升高和肌酸、肌酐的下降

造成肾小管上皮细胞水肿和空泡变性

肾小球个别出现瘀血的现象

同时引起肾脏内源性代谢物如胆碱

磷酸胆碱

苯丙氨酸

谷氨酰胺的减少和异亮氨酸

亮氨酸

缬氨酸

丙氨酸

乙酸

肌酸/肌酐的增加;而甘遂-甘草1: 4组并未出现明显的血浆生化指标改变

仅出现肾小球轻微瘀血及肾小管上皮细胞扩张的现象

同时仅引起胆碱

磷酸胆碱和苯丙氨酸的减少. 结论: 甘遂-甘草以1: 4配伍存在明显的减毒作用

主要是通过甘遂毒性生物标志物含量的变化和肾脏毒性传统指标的下调来体现其减毒效应.

Abstract

Objective: In this study

we investigated the impact of combining Kansui Radix with Glycyrrhizae Radix et Rhizoma(1: 4) on the kidney in rats. On this basis

the mechanism of the reducing toxicity was also studied. Method: Male Sprague-Dawley (SD) rats were randomly assign to four groups

which were controls

Kansui Radix

Glycyrrhizae Radix et Rhizoma and Kansui Radix-Glycyrrhizae Radix et Rhizoma(1: 4).The kidney extract metabonomic analyses of Kansui Radix

Glycyrrhizae Radix et Rhizoma and Kansui Radix-Glycyrrhizae Radix et Rhizoma(1: 4) was used by NMR and OPLS-DA. Clinical biochemical and histopathological assessments were also performed. Result: All the clinical biochemical

histopathological and metabolites results were no significant change in Glycyrrhizae Radix et Rhizoma treated rats. Not only the increased glucose and decreased creatine/creatinine in plasma

but also the renal tubular epithelial cell swelling and vacuoles degeneration

glomerular blood clot were observed in Kansui Radix treated rats. Besides

decreased choline

phosphorylcholine

phenylalanine and glutamine

increased isoleucine

leucine

alanine

valine

acetic acid

creatine/creatinine was observed in Kansui Radix treated rats. In Kansui Radix-Glycyrrhizae Radix et Rhizoma(1: 4)treatment group

there was no significant change in plasma index. The histopathological changes was only the glomerular congestion and renal tubular epithelial cells expansion. The variations were only declined of choline

phosphorylcholine and phenylalanine in Kansui Radix. Conclusion: Detoxification of Kansui Radix in kidney by combining with Glycyrrhizae Radix et Rhizoma was obvious. It could be owing to the intervention of the biomarker of Kansui Radix and down regulation of the traditional indicator of kidney lesion.

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