WANG Yu-jin, ZHOU Yong-xue, ZHANG Hong, et al. Effect of Xiaofu Tongjie Fang on Expression of VIP and AQP3 in Colon Tissue of Rats with Functional Constipation[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(9): 108-111.
DOI:
WANG Yu-jin, ZHOU Yong-xue, ZHANG Hong, et al. Effect of Xiaofu Tongjie Fang on Expression of VIP and AQP3 in Colon Tissue of Rats with Functional Constipation[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(9): 108-111. DOI: 10.13422/j.cnki.syfjx.2015090108.
Effect of Xiaofu Tongjie Fang on Expression of VIP and AQP3 in Colon Tissue of Rats with Functional Constipation
Objective: To explore the effect of Xiaofu Tongjie Fang (XFTJF) on expressions of vasoactive intestinal peptide (VIP) and aquaporin 3 (AQP3) in colon tissue of rats with functional constipation. Method: Fifty adult male SD rats were randomly divided into the control group
the model group
the high-
medium-and low-dose XFTJF groups (380
190
95 g·kg-1) of 10 rats in each group. The functional constipation model was induced by using compound diphenoxylate tablets (5 mg·kg-1) orally. The rats received intragastric administration of XFTJF for 3 weeks. The general performance
the time to first black stool and fecal water content were observed. The expressions of VIP and AQP3 in rat colon tissue were detected by using immunohistochemical method. The mRNA expression levels of VIP and AQP3 in colon tissue were further detected by using RT-qPCR. Result: Compared with the control group
the time to first black stool significantly prolonged
fecal water content reduced
VIP mRNA level decreased
and AQP3 mRNA level increased in the model group (P <0.05
P <0.01). Compared with the model group
the time to first black stool shortened
fecal water content increased
VIP mRNA level increased
and AQP3 mRNA level decreased in all XFTJF groups (P <0.05
P <0.01). Conclusion: The occurrence of functional constipation in rats is probably related to the abnormal expressions of VIP and AQP3 in colon tissue. The mechanism of XFTJF in the treatment of functional constipation may be realized through regulating the expressions of VIP and AQP3 in colonic tissue.
Effect of Xiaofu Tongjie Fang on Functional Constipation Rats Defecation Function and Gastrointestinal Motility
Effect of Combined Therapy of Lung and Intestine on VIP and p38 MAPK Signaling Pathways in Bronchial Asthma Mice
Clinical Observation of Modified Buzhong Yiqitang Combined with Erxian Decoction in Treatment of Stress Urinary Incontinence in Perimenopausal Women
Clinical Efficacy of Modified Chaihu Shugansan Combined with Xuanfu Daizhetang on Depression of Patients of Patients with Gastroesophageal Reflux Disease
Clinical Efficacy and 5-HT, VIP Levels of Hegan Granule in Treating Non-erosive Reflux Disease of Liver Depression and Spleen Deficiency
Related Author
LI Jing-tao
SHI Jie
YAN Shu-guang
ZHENG Xu-rui
ZHANG Xin-yue
HUI Yi
Xiao-yun YAO
Ting-ting ZHAI
Related Institution
College of Basic Medicine,Shaaxi University of Chinese Medicine
Affiliated Hospital,Shaaxi University of Chinese Medicine
The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine(TCM)
The Second Affiliated Hospital of Guizhou University of TCM