LI Guo-wei, WU Cheng-feng, GUI Shu-hua. Effect of Compatibility on Aconitine Type Alkaloids in Wutou Tang[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 5-8.
DOI:
LI Guo-wei, WU Cheng-feng, GUI Shu-hua. Effect of Compatibility on Aconitine Type Alkaloids in Wutou Tang[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 5-8. DOI: 10.13422/j.cnki.syfjx.2015100005.
Effect of Compatibility on Aconitine Type Alkaloids in Wutou Tang
Objective: To investigate effect of compatibility on alkaloids in Aconiti Radix Cocta from Wutou Tang. Method: HPLC-ESI-MS positive ion mode was adopted to analyze alkaloids in Aconiti Radix Cocta single decoction and Wutou Tang
parsing each component of MS2 and MS3 spectra
thus comparing alkaloids between Aconiti Radix Cocta single decoction and Wutou Tang. Result: Thirty-six common compositions were detected in Aconiti Radix Cocta single decoction and Wutou Tang
13 ingredients were detected in single decoction
which were not detected in Wutou Tang
such as dehydrared 10-OH-aconitine
dehydrated 10-OH-aconine
mesaconitine and so on. 10-OH-mesaconine in Wutou Tang was not detected in single decoction solution of Aconiti Radix Cocta. Conclusion: A lot of aconitine type alkaloids in Aconiti Radix Cocta are missing after compatibility
ingredients are clearly different.This established HPLC-ESI-MS can effectively separate various aconitine type alkaloids by providing molecular weight and structure information of components.
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