HUANG Ting-zhang, GUO Sheng-qi, QI Liang-yu, et al. Investigation of Antioxidant Activity of Eriocauli Flos Extract[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 13-15.
DOI:
HUANG Ting-zhang, GUO Sheng-qi, QI Liang-yu, et al. Investigation of Antioxidant Activity of Eriocauli Flos Extract[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 13-15. DOI: 10.13422/j.cnki.syfjx.2015100013.
Investigation of Antioxidant Activity of Eriocauli Flos Extract
Objective: To investigate antioxidant activity of water extract and alcohol extract of Eriocauli Flos. Method: Scavenging effect of water extract and alcohol extract of Eriocauli Flos on superoxide anion free radical
DPPH radical and hydroxyl radical were determined by UV method
reduction ability of extract for Fe3+ was determined by Prussian law
and with butylated hydroxyanisole (BHA) as reference
chelating ability of extract on metal ferrous ion (Fe2+) was determined. Result: Water extract and alcohol extract of Eriocauli Flos had strong scavenging effects on three kinds of free radicals
half inhibitory concentrations (IC50) of water extract were 1.389
3.507
0.341 g ·L-1
while IC50 of alcohol extract were 0.792
5.525
0.192 g ·L-1.Extract had reduction ability on Fe3+
and had a strong chelating ability to Fe2+
effects increased with increasing of extract concentration
which showed corresponding dose-response relationship. Conclusion: Water extract and alcohol extract of Eriocauli Flos have strong antioxidant activity
and at a relatively low concentration
alcohol extract is stronger than water extract about antioxidant effect.
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