WU Gui-fang, FENG Su-xiang, HUANG Tian-shan, et al. Optimization of Extraction Process for Paozhenkang Spray[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 30-33.
DOI:
WU Gui-fang, FENG Su-xiang, HUANG Tian-shan, et al. Optimization of Extraction Process for Paozhenkang Spray[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 30-33. DOI: 10.13422/j.cnki.syfjx.2015100030.
Optimization of Extraction Process for Paozhenkang Spray
Objective: To optimize extraction process of Paozhenkang spray. Method: Taking composite score of the content of berberine hydrochloride and dry extract rate as index
orthogonal test was adopted to investigate influence of extraction time
extraction times
the amount of water on water extraction process.With composite score of the total content of aloe-emodin
rhein
emodin
chrysophanol
physcion and dry extract rate as index
orthogonal test was adopted to optimize alcohol extraction process by taking ethanol concentration
alcohol amount
extraction time and times as factors.Contents of berberine hydrochloride and five aglycones from Rhei Radix et Rhizoma were determined by HPLC. Result: Optimun water extraction process consisted of adding 12-fold of waters
decocting 3 times
90 min for each time
the content of berberine hydrochloride was 6.07%.Optimun alcohol extraction process was as following:refluxing extracted twice with 10-fold of 75% ethanol
75 min for each time;the total content of five aglycones was 1.96%. Conclusion: These optimized extraction processes are stable and feasible for industrial production of Paozhenkang spray.
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Related Author
Ni YAO
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Xitong ZHAO
Yang CHEN
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Jun GUAN
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Related Institution
School of Chinese Materia Medica, Beijing University of Chinese Medicine
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