Objective: To explore relationship between the content of total polysaccharides from Pseudostellariae Radix and altitude by determining height above sea level with the growth area in Guizhou

and provide theory foundation for quality assessment and scientific cultivation of Pseudostellariae Radix. Method: Ultraviolet speetrophotometry was used to dertermine the content of total polysaccharides

total polysaccharides from Pseudostellariae Radix was extracted by water extraction and alcohol precipitation method

sulfuric acid-anthrone was used as sprayed regents and detection wavelength was at 626 nm.Altitudes were determined by global positioning system. Result: The content of total polysaccharides in Pseudostellariae Radix varied significantly in different altitude

it was the highest of 281 mg · g-1 from Shibing county cattle big dam small toward the old

but it was the lowest of 23 mg · g-1 from Luotian

Wangzhuang

Qingzhen city.But the content of total polysaccharides had no relationship with altitude. Conclusion: The content of total polysaccharides in Pseudostellariae Radix from Guizhou is generally high

altitude has no significant effect on the content of total polysaccharides

but high altitude area should be avoided.Although Guizhou is not famous-region for Pseudostellariae Radix

it can be builded as main planting base.