ZHU Kai-xin, SU Ben-wei, ZHAO Ming-hui, et al. Correlation Analysis of Oleanolic Acid and Ursolic Acid Between and its Eleven Kinds of Taxilli Herba[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 55-58.
DOI:
ZHU Kai-xin, SU Ben-wei, ZHAO Ming-hui, et al. Correlation Analysis of Oleanolic Acid and Ursolic Acid Between and its Eleven Kinds of Taxilli Herba[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 55-58. DOI: 10.13422/j.cnki.syfjx.2015100055.
Correlation Analysis of Oleanolic Acid and Ursolic Acid Between and its Eleven Kinds of Taxilli Herba
Objective: To analyze the component correlation of oleanolic acid and ursolic acid between Diospyros kaki and its eleven kinds of Taxilli Herba. Method: Oleanolic acid and ursolic acid were extracted with ethanol by ultrasound for 30 minutes
and determined by RP-HPLC. Agilent Eclipse XDB C18column (4.6 mm×250 mm
5 μm) was used for the separation
with a flow rate of 1.0 mL · min-1 and column temperature of 25 ℃. The mobile phase were methanol-acetonitrile-0.05% solution of ammonium acetate (12:67:21) and detection wavelength was 210 nm. Result: There were good linearity in the range of 2.34-375 mg · L-1(r=0.999 9) and 5.21-834 mg · L-1(r=0.999 9) for oleanolic acid and ursolic acid
respectively. The average recovery rates were 99.57% and 99.72% for oleanolic acid and ursolic acid
respectively. In D. kaki
the content of oleanolic acid was 0.382 6-0.541 1 mg · g-1(branches) and 1.773 6-4.274 6 mg ·g-1 (leaves)
and ursolic acid was 0.649 0-1.625 8 mg · g-1 (branches) and 6.023 7-9.398 0 mg · g-1(leaves). Ursolic acid was not detected in the eleven kinds of Taxilli Herba
and oleanolic acid was found only in Viscum ovalifolium and V. diospyrosicolum. The contents of oleanolic acid were 1.221 4 mg · g-1(in branches of V. ovalifolium)
10.815 8 mg · g-1 (in leaves of V. ovalifolium)
and 7.158 4 mg · g-1 (in V. diospyrosicolum)
respectively. Conclusion: The oleanolic acid and ursolic acid were accumulated in the branches and leaves of D. kaki. Oleanolic acid was accumulated in V. ovalifolium and V. diospyrosicolum
but ursolic acid was not accumulated. The oleanolic acid and ursolic acid were not accumulated in Macrosolen cochinchinensis