XIE Cai-xia, XIE Hui-Ying, BAI Yan, et al. Rapid Determination of Mangiferin in Anemarrhenae Rhizoma by Near-infrared Spectroscopy Technique[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 59-62.
DOI:
XIE Cai-xia, XIE Hui-Ying, BAI Yan, et al. Rapid Determination of Mangiferin in Anemarrhenae Rhizoma by Near-infrared Spectroscopy Technique[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 59-62. DOI: 10.13422/j.cnki.syfjx.2015100059.
Rapid Determination of Mangiferin in Anemarrhenae Rhizoma by Near-infrared Spectroscopy Technique
Objective: Determine the content of mangiferin in Anemarrhenae Rhizoma by near-infrared spectroscopy(NIRS). Method: Use the content of mangiferin in samples determined by HPLC as reference value. The quantitative model of mangiferin in Anemarrhenae Rhizoma was established by NIRS combining with partial least squares(PLS). Result: The correlation coefficients (R2) was 0.963 61
the root mean square error of calibration (RMSEC) was 0.061 6
the root mean square error of predication (RMSEP) was 0.088 1. Conclusion: The method is accurate
fast
simple. It can be used as a rapid detection method for Anemarrhenae Rhizoma.
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