Objective: The contents of baicalin and chlorogenic acid in Lidan tablets were simultaneously determined by HPLC. Method: The sample were separated on Capcell Pak C18 (4.6 mm×150 mm

5 μm) column

which eluted with acetonitrile (A) and 0.3% phosphoric acid (B). The ratio of acetonitrile and water increased from 87:13 to 67:33 in 20 min with detected wavelength at 315 nm

flow rate at 1 mL · min-1

column temperature at 30 ℃ and injection volume was 10 μL. Result: The result showed that two compounds reach the baseline separation

the linear range of baicalin and chlorogenic acid was 0.001 0-0.049 9 g · L-1 (r=0.999 9) and 0.005 3-0.026 7 g · L-1 (r=0.999 3)

respectively

and the average recovery of baicalin and chlorogenic acid was 98.62% (RSD 1.1%) and 98.98% (RSD 0.3%)

respectively. Conclusion: The method is rapid

precise and practical that it can be used to determine the two components in Lidan tablets.