Determination of Oleanolic Acid and Ursolic Acid in Lycopi Herba from Different Regions by HPLC

YAO Jing

doi:10.13422/j.cnki.syfjx.2015100080

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Determination of Oleanolic Acid and Ursolic Acid in Lycopi Herba from Different Regions by HPLC

更新时间：2024-01-04

- Determination of Oleanolic Acid and Ursolic Acid in Lycopi Herba from Different Regions by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 10, Pages: 80-82(2015)
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- DOI：10.13422/j.cnki.syfjx.2015100080
  CLC： O657.72;R284.1
- Published：2015
- 稿件说明：
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YAO Jing. Determination of Oleanolic Acid and Ursolic Acid in Lycopi Herba from Different Regions by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 80-82.
DOI：

YAO Jing. Determination of Oleanolic Acid and Ursolic Acid in Lycopi Herba from Different Regions by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 80-82. DOI： 10.13422/j.cnki.syfjx.2015100080.

摘要

目的: 建立泽兰药材中齐墩果酸和熊果酸的含量测定方法

并对收集自不同产地(海拔15~2 750 m)的泽兰药材进行含量测定

寻找海拔高度与药材质量的关系. 方法: 采用AgilentTC C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-乙腈-0.2%冰乙酸(78:10:12)

流速0.8 mL ·min-1

在210 nm处检测

柱温40 ℃. 结果: 齐墩果酸和熊果酸的的线性范围分别为0.164~2.46

0.325 6~4.884 μg

回归方程分别为Y=5.025×105X+2.106×103(r=0.999 8)和Y=4.655×105X+1.013×104(r=0.999 8)

平均加样回收率分别为99.03%(RSD 1.7%)和98.83%(RSD 1.4%).海拔高度与药材质量呈现一定的正相关性

即海拔越高

泽兰药材中齐墩果酸和熊果酸含量也越高. 结论: 所建立的含量测定方法简便快速、准确可靠

可用于评价泽兰药材的质量.海拔高度与药材质量的关系可指导泽兰药材种植区域的优选.

Abstract

Objective: To set up an content determination method for oleanolic acid and ursolic acid in Lycopi Herba by HPLC

and to find the relationship between altitude and medicinal material content. Method: AgilentTC C18 column(4.6 mm×250 mm

5 μm) was selected

the mobile phase was methanol-acetonitrile-0.2% glacial acetic acid solution(78:10:12)

the flow rate was 0.8 mL · min-1. The column temperature performed at 40 ℃

and the target component was detected at 210 nm. Result: Oleanolic acid showed a linear ranges at 0.164-2.46 μg(r=0.999 8) and ursolic acid was 0.325 6-4.884 μg(r=0.999 8). The average recovery of the added sample were 99.03% (RSD 1.7%) and 98.83% (RSD 1.4%)

respectively. Altitude and herbal content showed a certain degree of positive correlation. Conclusion: The method is simple and rapid

accurate and reliable

therefore available for the elevation of Lycopi Herba. Relationship between altitude and quality can be a preferably guidance for planting area.

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Related Institution

Jiangxi College of Traditional Chinese Medicine

Dept.of Pharmacy,The First Affiliated Hospital of Xinjiang Medical University

Tianjin University of Traditional Chinese Medicine

Tianjin Insititute of Pharmaceutical

Anhui College of Traditional Chinese Medicine,Anhui Key Laboratory of Modernized Chinese Medicine

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