Inflammation Effect of Yuanhuacine Induced on Macrophage by UPLC-MS/MS

YAN Wen-li; MA Hong-yue; WU De-kang; GONG Yan; WANG Zi-yue; CHEN Yan-yan; DUAN Jin-ao; ZHOU Jing; WANG Hong-lan; ZHANG Jun-feng; TONG Shu-juan

doi:10.13422/j.cnki.syfjx.2015100094

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Inflammation Effect of Yuanhuacine Induced on Macrophage by UPLC-MS/MS

更新时间：2024-01-04

- Inflammation Effect of Yuanhuacine Induced on Macrophage by UPLC-MS/MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 10, Pages: 94-98(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015100094
  CLC： R285
- Published：2015
- 稿件说明：
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YAN Wen-li, MA Hong-yue, WU De-kang, et al. Inflammation Effect of Yuanhuacine Induced on Macrophage by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 94-98.
DOI：

YAN Wen-li, MA Hong-yue, WU De-kang, et al. Inflammation Effect of Yuanhuacine Induced on Macrophage by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 94-98. DOI： 10.13422/j.cnki.syfjx.2015100094.

摘要

目的: 研究细胞上清中6种炎症介质花生四烯酸 (AA)

白三烯B4(LTB4)

前列腺素G2(PGG2)

前列腺素F2α(PGF2α)

15-脱氧-δ-12

14-PGJ2(15d-PGJ2)

15-过氧化氢二十碳四烯酸(5

15-DiHETE)的变化

探讨芫花酯甲干预花生四烯酸代谢产生的致炎、诱导平滑肌收缩的作用机制. 方法: 选取RAW264.7作为体外实验模型

采用三重四极杆质谱

分析空白组及低、高质量浓度组芫花酯甲(0

1.62

6.48 mg ·L-1)作用于RAW264.7细胞(5×104个/mL)48 h后细胞上清中6种炎症相关介质的相对含量变化

以探讨芫花酯甲的致炎机制. 结果: 与空白组相比

PGF2α

PGG2

15-DiHETE的相对含量显著升高(P<0.05);与空白组相比较

LTB4在高浓度芫花酯甲刺激时相对含量显著升高

但对于15d-PGJ2

高浓度的芫花酯甲刺激时相对含量呈现下降的趋势(P<0.01). 结论: 芫花酯甲增加了巨噬细胞上清液中炎症相关介质AA

15-DiHETE 及促炎介质LTB4

PGG2

PGF2α水平

减低了抗炎介质15d-PGJ2的水平

提示芫花酯甲通过影响促炎介质和抗炎介质的双重途径

发挥致炎和诱导平滑肌收缩作用.

Abstract

Objective: Effects of yuanhuacine on inflammatory related factors has been studied in cell supernatants. Method: Yuanhuacine(0

1.62

6.48 mg · L-1) was acted on RAW264.7 cells for 48h

the levels of inflammation-related mediators like arachidonic acid(AA)prostaglandin G2(PGG2)

prostaglandin F2α(PGF2α)

leukotriene B4(LTB4)

15-deoxy-δ-12

14-prostaglandin J2(15d-PGJ2)

15-dihydroxyeicosatetraenoic acid(5

15-DiHETE) were determined by UPLC-MS/MS. Result: Compared with the control group

the relative contents of AA

PGF2α

PGG2 and 5

15 DiHETE were markedly increased

and the relative content of LTB4 was also increased at the high dose of Yuanhuacine while the relative content of 15d-PGJ2 was decreased. Conclusion: Yuanhuacine can play a role in regulating both the proinflammatory and anti-inflammatory pathways.

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