Objective: To explore the effects of extracts from Ginseng Radix et Rhizoma

Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma on vascular endothelial cells and cadherin in replicative senescence of human cardiac microvascular endothelial cells (HCMECs) model. Method: The replicative senescence HCMECs model was induced in HCMECs by generation cultivating to the eighth cells. The in vitro cultured HCMECs were divided into 5 groups:the eighth generation cell-senescence untreated group (old group)

the resveratrol group (10 μmol · L-1)

the low-

intermediate-and high-dose herbal treated groups (50

100

200 mg · L-1). The intervention time of drug was 24 h. Changes of HUVECs aging were observed by the senescence-associated β-galactosidase staining (SA-β-gal)

cell proliferation was detected by using cell counting kit-8 (CCK-8)

and cell cycle was analyzed by using flow cytometry. The morphological changes of VE-cadherin were observed by using laser confocal microscopy. The protein expression of VE-cadherin was observed by using Western blot. Result: Compared with the old group

the extracts from Ginseng Radix et Rhizoma

Notoginseng Radix et Rhizoma and Chanxiong Rhizoma could decrease the proportion of aging stained

promote cell proliferation

reduce the proportion of G0/G1 phase

increase the proportion of G2/M phase

maintain the integrity of VE-cadherin

and promote the expression of VE-cadherin protein (P<0.05

P<0.01). Conclusion: The extracts from Ginseng Radix et Rhizoma

Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma could delay aging

promote the proliferation and mitosis

maintain the integrity of VE-cadherin and promote the expression of VE-cadherin protein in replicative senescence of HCMEC model

which may contribute to the delay of in vitro HCMEC senescence for Chinese herbal medicines of nourishing qi and resolving blood stasis.