Objective: To establish determination method for in vitro dissolution of alkaloids in Xianglian pills

reveal its in vitro dissloution behavior and influence factors. Method: HPLC was used to determine contents of berberine hydrochloride and palmatine hydrochloride with mobile phase of acetonitrile-potassium dihydrogen phosphate buffer (30 : 70) and detection wavelength at 350 nm.Small cup method was used to determine and draw dissloution curves of berberine hydrochloride and palmatine hydrochloride in Xianglian pills under different pH and volume of dissolution medium

rotation speed of stirring paddle.Origin Pro 8.0 was used to fit dissolution curves with Weibull distribution model and calculate dissolution parameters of T50 (the time for dissolution 50%) and Td (the time for dissolution 63.2%). Result: Dissloution curves of berberine hydrochloride and palmatine hydrochloride all fitted successfully with Weibull equation.These two components dissoved quickly and completely in dissolution medium with pH of 1.0

with increasing of pH of dissolution medium

both dissolution speed and rate decreased.When pH were 6.8 and 7.8

cumulative dissolution rate of these two components was less than 50%.With increasing of stirring speed

T50 and Tdof these two components slightly decreased.In volume range of 100-250 mL

both T50 and Td of these two components had not significant difference.Under the same conditions

T50 and Td of palmatine hydrochloride were less than these two parameters of berberine hydrochloride. Conclusion: Dissolution medium pH has significant influence on dissloution of alkaloids in Xianglian pills

rotation speed of stirring paddle has a certain effect on dissolution speed

but dissolution medium volume almost has no effect.Dissloution of palmatine hydrochloride is slightly quicker than berberine hydrochloride.