YAN Guang-jun, FAN Jian-wei, SU Rui-qiang, et al. HPLC Fingerprint Analysis of Organic Acids in Gardeniae Fructus at Different Collecting Stages[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 39-41.
DOI:
YAN Guang-jun, FAN Jian-wei, SU Rui-qiang, et al. HPLC Fingerprint Analysis of Organic Acids in Gardeniae Fructus at Different Collecting Stages[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 39-41. DOI: 10.13422/j.cnki.syfjx.2015110039.
HPLC Fingerprint Analysis of Organic Acids in Gardeniae Fructus at Different Collecting Stages
Objective: To compare HPLC fingerprints of organic acids in Gardeniae Fructus at different collecting stages for determining the dynamic changes of these compounds. Method: The chromatographic separations were obtained on a Kromasil C18 column (4.6 mm×250 mm
5 μm) with a gradient elution using the mobile phase consisting of 0.05% trifluoroacetic acid aqueous solution and acetonitrile at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 327 nm
and the column temperature was 40 ℃. The HPLC fingerprints were analyzed by similarity evaluation analysis to conduct the chemical pattern recognition. Result: Using the established reference fingerprint of Gardeniae Fructus as standard
all sample fingerprints for green
yellow and red Gardeniae Fructus were evaluated
with the ranges of similarity 0.94-1.00
0.81-0.92 and 0.53-0.74
respectively. Conclusion: The study offers the basis of quality control of Gardeniae Fructus and the realization of good agricultural practice.
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Related Institution
Henan Key Laboratory for Modern Research on Zhongjing's Herbal Formulae,Academy of Chinese Medical Sciences,Henan University of Chinese Medicine
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