QIN Chui-xin, CAO Zi-feng, HUANG Qi-min, et al. Pre-column Derivatives HPLC Fingerprint of Polygonati Rhizoma Polysaccharide Hydrolysate[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 65-68.
DOI:
QIN Chui-xin, CAO Zi-feng, HUANG Qi-min, et al. Pre-column Derivatives HPLC Fingerprint of Polygonati Rhizoma Polysaccharide Hydrolysate[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 65-68. DOI: 10.13422/j.cnki.syfjx.2015110065.
Pre-column Derivatives HPLC Fingerprint of Polygonati Rhizoma Polysaccharide Hydrolysate
采用HPLC测定单糖的衍生物.采用ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm
5 μm)
流动相0.1 mol·L-1磷酸缓冲液(pH 6.7)-乙腈(84.5 :15.5)
检测波长250 nm
柱温30 ℃
流速0.8 mL·min-1. 结果: 黄精多糖柱前衍生指纹图谱标示出10个共有峰
鉴别了7个共有峰(D-甘露糖
D-半乳糖醛酸
L-鼠李糖
D-半乳糖
D-葡萄糖
D-木糖
D-阿拉伯糖).10批黄精药材指纹图谱相似度 >0.99. 结论: 该方法简便
分离度高
重复性及稳定性良好
可有效控制黄精多糖的质量.
Abstract
Objective: To establish a pre-column derivation HPLC method of chromatographic fingerprint of Polygonati Rhizoma polysaccharide. Method: Polygonati Rhizoma polysaccharide was hydrolyzed with trifluoroacetic acid and derivated by 1-phenyl-3-methyl-5-pyrazolone (PMP). The monosaccharide composition was separated by reversed-phase technique on a Zorbax Eclipse XDB-C18 column (4.6 mm×250 mm
5 μm) with a mobile phase composed of 0.1 mol·L-1phosphate buffer (pH 6.7) and acetonitrile in the ratio of 84.5 : 15.5.The flow rate was 0.8 mL·min-1. The detection wavelength was set at 250 nm
and column temperature was at 30 ℃. Result: There were 10 common peaks from the fingerprint and 7 peaks were identified as D-mannose
L-rhamnose
D-galacturonic
D-galactose
D-glucose
D-xylose
and D-arabinose. The similarity of 10 batches of Polygonati Rhizoma polysaccharide was more than 0.99. Conclusion: The method is accurate and reproducible
which can be used for the quality control of Polygonati Rhizoma.
Approach Based on PMP-HPLC Fingerprint and Chemometrics to Compare Difference of Polysaccharide in Polygonati Rhizoma
Quantitative Fingerprint Chromatogram of
HPLC Fingerprint Chromatogram of Yinhuang Series Preparations
Progress in Study on Processing of Polygonati Rhizome in Past Decade
Multi-component Fingerprint Chromatogram of Shenxuebao Mixture by HPLC
Related Author
Ze-fei DU
Ai-en TAO
Cong-long XIA
Long-xing LI
Bao-zhong DUAN
WANG Xiao-xun
ZHANG Shou-ping
XIN Hua
Related Institution
College of Pharmaceutical science, Dali University
School of Pharmacy,Traditional Chinese Medicine(TCM) Processing Inheritance Base of National Administration of TCM,Ministry of Education-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials,Anhui Key Laboratory of New Technology for Manufacturing TCM Decoction Pieces,Anhui University of Chinese Medicine
Key Laboratory of Quality Research and Evaluation of TCM of National Medical Products Administration,Anhui Institute for Food and Drug Control
Jiangxi University of Chinese Medicine
Jiangxi Jingde Chinese Traditional Medicine Co. Ltd.