Objective: To investigate the effect of aconite containing serum on L-type calcium channel in isolated rat ventricular myocytes. Method: Aconite decoction 15.6 g·kg-1 was were ig given rats 7 d to prepare containing serum;The concentration of containing drug serum was 10% to obtain rat ventricular myocyte

enzymatic dissociation was used

whole cell patch clamp technique was used to record the L-type calcium channel current

L-type calcium channel current amplitude and kinetics characteristics were observed. Result: In the blank serum group and drug serum group the average peak current density and average activation after administration were respectively (-3.59±1.06) pA/pF

12.87%(n=8

P <0.05)and (-4.01±0.88) pA/pF

51.17%(n=8

P <0.01). In aconite containing serum before and after the treatment V1/2 and K of activation curve were respectively (-12.66±0.44) mV and (5.70±0.35);(-14.71±0.88) mV and (5.68±0.72)(P <0.05

n=8).In aconite containing serum before and after the treatment V1/2 and K of inactivation curve were respectively (-28.10±0.20) mV

and (6.31±0.18) mV;(-37.55±0.11) mV and (5.94±0.09) (P <0.05

n=8).Aconite containing serum recovery curves before and after medication for the τ:(171.01±11.13)ms and(160.15±4.97)ms(n=8). Conclusion: Aconite containing serum has activate function to the myocardial cells of L-type calcium channel currents.