Effect of  on Radiation-induced Lung Injury of Bama Minipigs

YUE Hai-ying; HU Kai; JIANG Jian; CHEN Yu-hua; LIU Wen-qi; WANG Ren-sheng

doi:10.13422/j.cnki.syfjx.2015110121

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Effect of on Radiation-induced Lung Injury of Bama Minipigs

更新时间：2024-01-04

- Effect of on Radiation-induced Lung Injury of Bama Minipigs
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 11, Pages: 121-126(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015110121
  CLC： R285.5
- Published：2015
- 稿件说明：
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YUE Hai-ying, HU Kai, JIANG Jian, et al. Effect of on Radiation-induced Lung Injury of Bama Minipigs[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 121-126.
DOI：

YUE Hai-ying, HU Kai, JIANG Jian, et al. Effect of on Radiation-induced Lung Injury of Bama Minipigs[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 121-126. DOI： 10.13422/j.cnki.syfjx.2015110121.

摘要

目的: 探讨肿节风对60COγ射线照射后在小型猪放射性肺损伤中的防护作用和机制. 方法: 60头雄性巴马小型猪随机分为正常组、照射组和肿节风组

在麻醉条件下对动物行右胸单次15 Gy的照射

正常组不予照射.肿节风组于放疗前1周开始经口腔给予肿节风配方颗粒溶液(30 mg·kg-1)

正常组和单照组给予等量的生理盐水

直至实验结束.在照射后4

24周

从3个组中分别随机取5头小型猪采集右肺组织

行羟脯氨酸测定检测肺组织中胶原含量;免疫印迹检测转化生长因子-β1(TGF-β1)

基质金属蛋白酶-2(MMP-2)和MMP-9的蛋白表达;明胶酶谱法测定MMP-2和MMP-9的酶活性. 结果: 在照射后不同时间点

照射组中羟脯氨酸含量

TGF-β1

MMP-2和MMP-9的蛋白表达

MMP-2

MMP-9的酶活性较正常组明显升高(P <0.05);与照射组比较

肿节风组的羟脯氨酸含量

TGF-β1

MMP-2和MMP-9的蛋白表达

MMP-2

MMP-9的酶活性明显降低

差异具有统计学意义(P <0.05). 结论: 肿节风可通过降低TGF-β1

MMP-2和MMP-9的蛋白表达和后者的酶活性

减缓放射性肺纤维化的发展

从而发挥放射防护作用.

Abstract

Objective: To evaluate the effect of Sarcandra glabra on radiation-induced lung injury of right thoracic irradiation of Bama minipigs. Method: Sixty miniature pigs were randomly divided into the control group

the radiotherapy group and the S. glabra group. The pigs except the control group received a single right thoracic irradiation under anesthesia. The pigs in the S. glabra group received S. glabra formula granule solution at 30 mg·kg-1 one week before radiotherapy

and the pigs in other groups received the same volume of normal saline..Five pigs were randomly selected and sacrificed from the three groups at week 4

12 and 24 post-irradiation. The collagen deposition was measured by Masson's trichrome and hydroxyproline detection. The protein expressions of transforming growth factor β1 (TGF-β1)

matrix metalloproteinase 2 (MMP-2) and MMP-9 were detected by Western Blot. The activities of MMP-2 and MMP-9 were tested by Gelatin zymography. Result: At different weeks after irradiation

the protein expressions of TGF-β1

MMP-2 and MMP-9

hydroxyproline content and gelatinase activities of MMP-2 and MMP-9 increased in the radiotherapy group as compare to the control group (P <0.05). The protein expressions of TGF-β1

MMP-2 and MMP-9

hydroxyproline content and gelatinase activities of MMP-2 and MMP-9 decreased in the S. glabra group as compared with the radiotherapy group (P <0.05). Conclusion: S. glabra could slow down the development of radiation pulmonary fibrosis and have radiological protection by reducing the protein of TGF-β1

MMP-2

MMP-9 and gelatinase activity.

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