Effects Zuogui Wan on mRNA Expression of 11 -HSD1 in Placenta of Gestational Diabetes Mellitus Rats

WANG Yue-yao; WANG Yong-hui; XU Kai-xia; CHAI Zhi; GAO Li; ZHOU Ran

doi:10.13422/j.cnki.syfjx.2015110127

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Effects Zuogui Wan on mRNA Expression of 11 -HSD1 in Placenta of Gestational Diabetes Mellitus Rats

更新时间：2024-01-04

- Effects Zuogui Wan on mRNA Expression of 11 -HSD1 in Placenta of Gestational Diabetes Mellitus Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 11, Pages: 127-130(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015110127
  CLC： R285.5
- Published：2015
- 稿件说明：
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WANG Yue-yao, WANG Yong-hui, XU Kai-xia, et al. Effects Zuogui Wan on mRNA Expression of 11 -HSD1 in Placenta of Gestational Diabetes Mellitus Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 127-130.
DOI：

WANG Yue-yao, WANG Yong-hui, XU Kai-xia, et al. Effects Zuogui Wan on mRNA Expression of 11 -HSD1 in Placenta of Gestational Diabetes Mellitus Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 127-130. DOI： 10.13422/j.cnki.syfjx.2015110127.

摘要

目的: 探讨左归丸对妊娠糖尿病大鼠胎盘组织11 β-羟基类固醇脱氢酶1(11 β-HSD1)基因表达水平的影响. 方法: 育龄雌性SD大鼠受孕后随机分为正常妊娠组(C)、妊娠糖尿组(GDM)、胰岛素干预组(INS)、左归丸干预组(ZGW)、左归丸联合胰岛素干预组(F)

每组10只;受孕当日GDM组和3个干预组按45 mg·kg-1 ip链脲佐菌素(STZ)复制妊娠糖尿病模型.并于孕4 d开始给药干预(左归丸10 g·kg-1 ig

胰岛素20 U·kg-1 ih).孕19 d断头取血

葡萄糖氧化酶法测定血糖;酶联免疫吸附法检测血清皮质酮、胰岛素;采用RT-qPCR技术检测胎盘11 β-HSD1基因mRNA的表达水平

并进行定量分析. 结果: 与正常妊娠组比较

GDM组血糖水平、血清胰岛素浓度、皮质酮水平、胎盘11 β-HSD1基因mRNA的表达水平均明显升高

差异有统计学意义(P <0.01);与妊娠糖尿组比较

左归丸干预组血糖水平、血清胰岛素浓度、皮质酮水平、胎盘11 β-HSD1基因mRNA的表达水平均明显降低(P <0.05

P <0.01)

但仍高于正常妊娠组

联合组治疗效果优于左归丸组. 结论: 左归丸可降低妊娠期糖尿病大鼠血糖水平

胎盘部位有11 β-HSD1基因的表达

妊娠糖尿病病理状态可影响11 β-HSD1基因在胎盘的表达水平

左归丸有下调11 β-HSD1基因表达水平的作用

推测可能是其改善GDM大鼠胰岛素抵抗的机制之一.

Abstract

Objective: To study the effects of Zuogui Wan on the mRNA expression of 11 β-hydroxysteroid dehydrogenase type 1(11 β-HSD1) in placenta of gestational diabetes mellitus (GDM) rats. Method: Pregnant reproduction age SD rats were randomly divided into 5 groups:the normal pregnancy (C) group

the GDM control (GDM) group

the diabetes insulin treated (INS) group

Zuogui Wan treated (ZGW) group and Zuogui Wan combined insulin treated (F) group. The GDM was induced except the C group by injecting streptozocin (STZ) abdominally at 45 mg·kg-1. The rats in the three treated groups received the treatment of Zuogui Wan and/or insulin from injecting STZ until 19-day pregnant. Glycemia was detected by using the glucose oxidase method. The corticosterone and insulin in blood serum were detected by using ELISA kit. The mRNA expression level of 11 β-HSD1 in placenta was evaluated by RT-qPCR

and the quantitative analysis was conducted. Result: Compared with the C group

the glycemia

serum insulin

corticosterone level and placental 11 β-HSD1 mRNA expression level increased in the GDM group (P <0.01). Compared with the GDM group

the glycemia level

serum insulin

corticosterone level and placental 11 β-HSD1 mRNA expression level decreased significantly in AGW and F groups

but the results were higher than those in the C group. Moreover

the effect is better in F group. Conclusion: Zuogui Wan could reduce the level of glycemia in diabetic rats. The mRNA expression of 11 β-HSD1 was found in placental and could be influenced by GDM. Zuogui Wans have the improving effects on the glycemic status of GDM rats

which may be related to reducing the placental 11 β-HSD1 gene expression level.

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