Effect of Kangnaoye on Expressions of BDNF, MMP-9, ERK1/2 on Cerebral Ischemia Reperfusion in Rats

MA Jian-peng; CHANG Qing; XUE Qian; ZOU Yu-an

doi:10.13422/j.cnki.syfjx.2015110131

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Effect of Kangnaoye on Expressions of BDNF, MMP-9, ERK1/2 on Cerebral Ischemia Reperfusion in Rats

更新时间：2024-01-04

- Effect of Kangnaoye on Expressions of BDNF, MMP-9, ERK1/2 on Cerebral Ischemia Reperfusion in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 11, Pages: 131-135(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015110131
  CLC： R285.5
- Published：2015
- 稿件说明：
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MA Jian-peng, CHANG Qing, XUE Qian, et al. Effect of Kangnaoye on Expressions of BDNF, MMP-9, ERK1/2 on Cerebral Ischemia Reperfusion in Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 131-135.
DOI：

MA Jian-peng, CHANG Qing, XUE Qian, et al. Effect of Kangnaoye on Expressions of BDNF, MMP-9, ERK1/2 on Cerebral Ischemia Reperfusion in Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 131-135. DOI： 10.13422/j.cnki.syfjx.2015110131.

摘要

目的: 通过康脑液干预观察其对脑缺血再灌注大鼠脑源性神经生长因子(BDNF)

基质金属蛋白酶-9(MMP-9)

细胞外信号调节激酶(ERK1/2)表达的影响

探讨康脑液对脑缺血的保护机制. 方法: 将雄性SD大鼠随机分为假手术组、脑缺血再灌注模型组(I/R)及康脑液高、中、低剂量(28.6

14.3

7.15 g·kg-1·d-1)组

线栓法制备大鼠右侧大脑中动脉阻塞(middle cerebral artery occlusion

MCAO)再灌注模型.于缺血2 h后再灌注(分别于再灌注后1

168 h后

处死大鼠)

用免疫组织化学法观察大鼠脑组织BDNF

MMP-9

ERK1/2的表达情况. 结果: 与I/R组比较

康脑液高、中剂量组各时间点的BDNF及ERK1/2表达量明显上调(P <0.05)

而MMP-9表达的阳性细胞明显减少(P <0.05);再灌注24 h梗死灶体积康脑液高

中剂量组较I/R组明显减小(P <0.05). 结论: 康脑液可促进大鼠局灶性脑缺血后脑组织中BDNF的表达

激活ERK1/2信号通路

同时抑制脑内MMP-9的表达

发挥对神经血管单元的保护作用.

Abstract

Objective: To observe the effect of Kangnaoye on the expression of brain derived neurotrophic factor (BDNF)

matrix metalloproteinase-9 (MMP-9)

extracellular signal regulated kinase 1/2 (ERK1/2) in rat with cerebral ischemia reperfusion injury

and to study its cerebral protection mechanism. Method: Male SD rats were randomly divided into the sham-operation group

the ischemia reperfusion (I/R) group

the high-

medium-

and low-dose Kangnaoye groups (28.6

14.3

7.15 g·kg-1·d-1). The cerebral ischemia reperfusion model was prepared in rats by a improved Zea Longa method. The rats were killed at 1-

12-

24-

72-and 168-h reperfusion after 2-h ischemia. The expression of BDNF

MMP-9 and ERK1/2 were observed by using immunohistochemical method. Result: Compared with I/R group

the expression of BDNF and ERK1/2 was upregulated

the numbers of MMP-9 positive cells decreased in the high-and medium-dose Kangnaoye groups (P <0.05). Moreover

the infarct volume decreased significantly at 24-reperfusion (P <0.05). Conclusion: Kangnaoye could protect neurovascular unit by promoting the expression of BDNF in brain tissue

activating ERK1/2 signaling pathway

and inhibiting the expression of MMP-9.

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