HAN Yi, YU Yan, WANG Xue-feng, et al. Optimization of Ethanol Extraction Process for Fuxiong Cataplasms by Multi-index Comprehensive Scoring Method[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(12): 5-8.
DOI:
HAN Yi, YU Yan, WANG Xue-feng, et al. Optimization of Ethanol Extraction Process for Fuxiong Cataplasms by Multi-index Comprehensive Scoring Method[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(12): 5-8. DOI: 10.13422/j.cnki.syfjx.2015120005.
Optimization of Ethanol Extraction Process for Fuxiong Cataplasms by Multi-index Comprehensive Scoring Method
Objective: To optimize ethanol extraction process of Fuxiong cataplasms. Method: With dry extract yield and contents of emodin
baicalin
morusin as indexes
orthogonal design was carried out for optimizing extraction process by taking the concentration and amount of ethanol
extracting time and times as factors
optimum process was determined by taking composite score for condition.Contents of emodin and baicalin were determined by HPLC
mobile phases were consisted of methanol-0.4% phosphoric acid (80: 20) and (47: 53)
detection wavelengths were 254 nm and 280 nm
respectively.The content of morusin was determined by UV spectrophotometry. Result: Optimum extraction technology was as follows:extracted twice with 6 times the amount of 75% ethanol for 3 h of each time.Dry extract yield and contents of emodin
baicalin
morusin were 31.19%
0.19%
3.61% and 0.51%
respectively. Conclusion: This optimized extraction technology is rational with high extraction rates of active components.
Simultaneous Determination of Baicalin and Emodin in Liyan Jiedu Granules by HPLC
Effect of Berberine-Baicalin Combination on Fecal Microbiota Transplantation-induced Type 2 Diabetes Mellitus Due to Internal Accumulation of Dampness-heat in Mice from Perspectives of Gut Microbiota and Metabolomics
Baicalin Induces Ferroptosis in Gastric Cancer Cells via p53-mediated SLC7A11 Down-regulation
Investigation of Effect of Polyethylene Glycol 400 on Pharmacokinetics and Anti-inflammatory Effect of Baicalin Based on UPLC-MS/MS and Molecular Docking Techniques
Inhibitory Effects of Ligustrazine Plus Emodin on Expression of VEGF-related Factors in Ascites Carcinoma Walker-256 Cells
Related Author
LIU A-jing
QI Guang-cai
LIU Zhen-ye
BAI Zi-jing
CHEN Mengjie
LIU Yimin
ZHOU Yun
YU Keming
Related Institution
Research Center for Differentiation and Development of Traditional Chinese Medicine (TCM) Basic Theory, Jiangxi Province Key Laboratory of TCM Etiopathogenisis, Jiangxi University of Chinese Medicine
Henan Academy of Chinese Medicine
The Second Clinical Medical College, Henan University of Chinese Medicine
State Key Laboratory of Functions and Applications of Medicinal Plants,School of Pharmaceutical Sciences,Engineering Center of Microbiology and Biochemical Pharmacy,Experimental Animal Center,Guizhou Medical University
The Second Affiliated Hospital of Air Force Medical University