Objective: To establish a dual-wavelength HPLC method for simultaneous determination of five constituents (chlorogenic acid

resveratrol

rutin

quercetin

kaempferol) in Jingangteng soft capsule. Method: The Agilent ZORBAX SB-C18 column (4.6 mm×150 mm

5 μm) with a column temperature of 25 ℃ was used;the mobile phase was acetonitrile-0.1% phosphoric acid at gradient elution program and with a flow rate of 1.0 mL·min-1

and the detection wavelengths were set at 306 nm (chlorogenic acid and resveratrol) and 365 nm(rutin

quercetin and kaempferol). Result: The linear response ranges of chlorogenic acid

resveratrol

rutin

quercetin

and kaempferol were 0.058 5-2.34 μg(r=0.999 8)

0.025 3-1.01 μg (r=0.999 9)

0.042 2 -0.844 μg (r=0.999 6)

0.018 1-0.722 μg(r=0.999 9)

and 0.016 5-0.660 μg (r=0.999 9)

respectively. The average recoveries were 98.0%

100.6%

96.0%

102.7%

and 98.5% with RSDs of 1.2%

2.2%

1.8%

2.9%

and 1.3%

respectively. Conclusion: The developed method was convenient and reliable

which could be applied to determination of five effective constituents in Jinggangteng soft capsule.