Objective: To establish fingerprint of total alkaloids from Isatidis Radix by HPLC. Method: An HPLC fingerprint has been established by gradient elution of acetonitrile and 0.1% H3PO4 aqueous solution

using a Phenomenex column(4.6 mm×250 mm

5 μm). Detection wavelength was set at 220 nm

Column temperature was not controlled

flow rate was 1.0 mL·min-1

injection volume was 10 μL. The fingerprints were compared with similarity evaluation software published by the 'Similarity Evaluation of Chromatographic Fingerprint of Traditional Chinese Medicine' (2004 A). Result: HPLC fingerprints of alkaloids were established through 12 batchesof Isatidis Radix

and 19 common peaks were identified. Conclusion: This method has been proven to be feasible for the identification of Isatidis Radix.The method is simple

specific

stable

and repeatable

which will provide a methodological basis of quality control of alkaloids from Isatidis Radix.