Objective: To investigate antiviral activity of Jinqiao Reduqing granules and Jinqiao Reduqing spray drying powder

and compare the difference of antiviral activity between them in vitro and in vivo. Method: In vitro experiment

the 3 -(4

5-Dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT)colorimetric method was applied to determine the 50% inhibitory concentration (IC50) against influenza virus FM1 of Jinqiao Reduqing granules and spray drying powder by different administration ways (drug was used directly on the virus or virus-infected cells). Therapy indexes (TI) of them were also calculated. The mice were randomly divided into control group

Ribavirin(0.07 g·kg-1)

Jinqiao Reduqing granules(11.7 g·kg-1)

Jinqiao Reduqing spray drying powder(5.85

11.7

23.4 g·kg-1). In vivo experiment

the mice were infected with influenza virus FM1 through nasal inhalation

the mice were intragastric administrated after infection virus. The life-extension rate

lung index of infected mice were observed to investigate the protective function of Jinqiao Reduqing spray drying powder and Jinqiao Reduqing granules against influenza virus. Result: For two different administration ways

the IC50 of Jinqiao Reduqing spray drying powder was 0.77

0.80 g·L-1

and the TI were 11.78

11.34 respectively. The IC50 of Jinqiao Reduqing granules were 3.70

3.60 g·L-1

and the TI were 6.37

6.55 respectively. At the dose of 23.4 g·kg-1 for Jinqiao Reduqing spray drying powder

its life-extension rate was 60.81%

and the lung index inhibition rate was 13.18%.Compared with the virus control group

the difference were statistically significant(P<0.05). Conclusion: Jinqiao Reduqing spray drying powder and Jinqiao Reduqing granules have anti-influenza virus activity. But at the same dose

the anti-influenza virus FM1 function of Jinqiao Reduqing spray drying powder was more powerful than that of Jinqiao Reduqing granules in vitro.