LI Xue, SHEN Min-qin. Effect of Changkang Formula on SP and VIP in Rats with Diarrhea-predominant Irritable Bowel Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(12): 112-115.
DOI:
LI Xue, SHEN Min-qin. Effect of Changkang Formula on SP and VIP in Rats with Diarrhea-predominant Irritable Bowel Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(12): 112-115. DOI: 10.13422/j.cnki.syfjx.2015120112.
Effect of Changkang Formula on SP and VIP in Rats with Diarrhea-predominant Irritable Bowel Syndrome
Objective: To study the effects of Changkang formula (CF) on plasma P substance (SP) and colon vasoactive intestinal peptide (VIP) in rats with diarrhea-predominant irritable bowel syndrome (D-IBS)
and to explore its possible mechanism on D-IBS. Method: Sixty Sprague-Dawley rats were randomly divided into 6 groups: the normal control group (normal saline)
model group (normal saline)
the maleate trimebutine group (15 mg·kg-1)
the low-
middle and high-dose CF groups (1.1
2.2
4.4 g·kg-1). The D-IBS model was induced in rats by senna-free drinking and stress-restrainting for 4 weeks. The corresponding medicines were intragastrically administered to rats once daily for 3 weeks. The abdominal withdrawal reflex (AWR) was scored. The levels of SP in serum and VIP in tissue were detected by using the ELISA assay. Result: Compared with the normal group
AWR score and plasma SP level increased
colon VIP level decreased in the model group (P<0.05
P<0.01). Compared with the model group
AWR score and plasma SP level decreased
colon VIP level increased in the low-
middle and high-dose CF groups (P<0.05
P<0.01). Conclusion: CF had a therapeutic effect on D-IBS by improving visceral hypersensitivity
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