Objective: To observe the direct protective effect of Cidan injection on isolated rat heart. Method: SD rats

half male and half female

were randomly divided into 4 groups: the control group (physiological saline

10 mL·L-1)

the Acanthopanax senticosus injection group (10 mL·L-1)

the Salvia miltiorrhiza injection group (10 mL·L-1) and the Cidan injection group (8 mL·L-1) of 8 rats each. The Langendorff heart perfusion in vitro model of 8 channels physiological signal recording system was used in this study. The change of left ventricular systolic pressure (LVSP)

left ventricular end-diastolic pressure (LVEDP)

left ventricular pressure (LVDP=LVSP-LVEDP)

left ventricular pressure maximum rising rate (+dp/dtmax)

left ventricular pressure maximum decline rate (-dp/dtmax) and heart rate (HR) were tested. The increasing amount of each index between Cidan injection group and Acanthopanax senticosus injection group

Salvia miltiorrhiza injection group was compared

respectively. The Cidan injection groups were then divided into 5 dose nutrition perfusate groups (1

2

4

8

16 mL·L-1)

and the dose effect relationship was investigated. Result: Compared with the control group

LVSP

LVDP

+dp/dtmax increased

HR

LVEDP and -dp/dtmax decreased in the Cidan injection group. There were significant differences for the increasing amount of each index as compared with the Acanthopanax injection and Salvia miltiorrhiza injection groups (P<0.05

P<0.01). Cidan injection showed a certain dose effect relationship within the dose of 1-8 mL·L-1

and it had the most significant efficacy at the dose of 8 mL·L-1. Conclusion: Cidan injection could slow the HR

improve cardiac systolic and diastolic function

and the effect is better than Acanthopanax senticosus injection and salvia miltiorrhiza injection. Cidan injection shows a dose-dependent effect in a certain range of dose.